Ding Linxian, Hirose Taketo, Yokota Akira
Laboratory of Bioresources, Institute of Molecular and Cellular Biosciences, The University of Tokyo, Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan.
Int J Syst Evol Microbiol. 2007 Aug;57(Pt 8):1747-1751. doi: 10.1099/ijs.0.64791-0.
The taxonomic position of two actinomycete strains, LC2(T) and LC11(T), isolated from a filtration substrate made from Japanese volcanic soil, was determined using a polyphasic approach. The strains grew at temperatures from 5 to 45 degrees C, on media of pH between 6 and 11 and in the presence of 7 % NaCl. The major menaquinone was MK-9(H(4)). The major fatty acid was iso-C(16 : 0). A phylogenetic tree based on 16S rRNA gene sequences showed that the two strains formed a distinct evolutionary lineage within the genus Amycolatopsis. On the basis of their morphological, physiological and genotypic characteristics, the isolates are proposed to represent two novel species of the genus Amycolatopsis, for which the names Amycolatopsis echigonensis sp. nov. (type strain LC2(T) =IAM 15387(T) =CCTCC AB206019(T)), and Amycolatopsis niigatensis sp. nov. (type strain LC11(T) =IAM 15388(T) =CCTCC AB206020(T)) are proposed.
采用多相分类法确定了从日本火山土壤制成的过滤基质中分离出的两株放线菌菌株LC2(T)和LC11(T)的分类地位。这些菌株在5至45摄氏度的温度下、pH值在6至11之间的培养基中以及存在7%氯化钠的条件下生长。主要甲基萘醌为MK-9(H(4))。主要脂肪酸为异-C(16 : 0)。基于16S rRNA基因序列构建的系统发育树表明，这两株菌株在拟无枝酸菌属内形成了一个独特的进化分支。根据其形态、生理和基因型特征，提议将这些分离物代表拟无枝酸菌属的两个新物种，分别命名为江户拟无枝酸菌（Amycolatopsis echigonensis sp. nov.）（模式菌株LC2(T)=IAM 15387(T)=CCTCC AB206019(T)）和新泻拟无枝酸菌（Amycolatopsis niigatensis sp. nov.）（模式菌株LC11(T)=IAM 15388(T)=CCTCC AB206020(T)）。
