Yoon Min-Ho, Ten Leonid N, Im Wan-Taek
Department of Bio-Environmental Chemistry, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 305-764, Republic of Korea.
Department of Biology and Medicinal Sciences, Pai Chai University, Daejeon 302-735, Republic of Korea.
Int J Syst Evol Microbiol. 2007 Aug;57(Pt 8):1810-1814. doi: 10.1099/ijs.0.64906-0.
A Gram-positive, aerobic, non-motile, rod-shaped, spore-forming bacterium, designated Gsoil 139(T), was isolated from soil from a ginseng field in Pocheon Province, South Korea, and was characterized using a polyphasic approach. Comparative analysis of 16S rRNA gene sequences revealed that strain Gsoil 139(T) belongs to the family Paenibacillaceae. The greatest sequence similarity was found with respect to the type strains of Paenibacillus hodogayensis (95.6 %) and Paenibacillus koleovorans (93.8 %). The strain showed less than 93.8 % sequence similarity with respect to other species of the genus Paenibacillus. The G+C content of the genomic DNA was 48.1 mol%. In addition, the presence of MK-7 as the major menaquinone and C(15 : 0) anteiso as a major fatty acid (27.9 %) justifies its affiliation to the genus Paenibacillus. On the basis of its phenotypic characteristics and phylogenetic distinctiveness, strain Gsoil 139(T) represents a novel species within the genus Paenibacillus, for which the name Paenibacillus ginsengarvi sp. nov. is proposed. The type strain is Gsoil 139(T) (=KCTC 13059(T) =DSM 18677(T)).
从韩国抱川市人参田土壤中分离出一株革兰氏阳性、需氧、不运动、杆状、产芽孢细菌，命名为Gsoil 139(T)，并采用多相分类法对其进行了鉴定。16S rRNA基因序列的比较分析表明，菌株Gsoil 139(T)属于类芽孢杆菌科。与保土类芽孢杆菌（Paenibacillus hodogayensis）模式菌株（95.6%）和食油类芽孢杆菌（Paenibacillus koleovorans）模式菌株（93.8%）的序列相似性最高。该菌株与类芽孢杆菌属其他种的序列相似性低于93.8%。基因组DNA的G+C含量为48.1 mol%。此外，以MK-7作为主要甲基萘醌和anteiso-C(15 : 0)作为主要脂肪酸（27.9%），证明其属于类芽孢杆菌属。基于其表型特征和系统发育独特性，菌株Gsoil 139(T)代表类芽孢杆菌属内的一个新种，为此提出新种名为人参类芽孢杆菌（Paenibacillus ginsengarvi sp. nov.）。模式菌株为Gsoil 139(T)（=KCTC 13059(T)=DSM 18677(T)）。
