Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1, Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea.
Department of Oriental Medicinal Material and Processing, College of Life Science, Kyung Hee University, 1 Seocheon-dong, Yongin-si, Gyeonggi-do 446-701, Republic of Korea.
Int J Syst Evol Microbiol. 2010 May;60(Pt 5):1163-1167. doi: 10.1099/ijs.0.009548-0. Epub 2009 Aug 10.
A Gram-positive, aerobic or facultatively anaerobic, rod-shaped, spore-forming bacterium, strain Gsoil 1138(T), was isolated from soil of a ginseng field in Pocheon Province, South Korea, and was characterized in order to determine its taxonomic position. On the basis of 16S rRNA gene sequence analysis, strain Gsoil 1138(T) was shown to belong to the family Paenibacillaceae and was most closely related to the type strains of Paenibacillus chondroitinus (98.2 % similarity) and Paenibacillus alginolyticus (96.5 %). Levels of 16S rRNA gene sequence similarity between strain Gsoil 1138(T) and the type strains of other recognized species of the genus Paenibacillus were below 96.5 %. The G+C content of the genomic DNA of strain Gsoil 1138(T) was 52.1+/-0.2 mol% (mean+/-sd of three determinations). Phenotypic and chemotaxonomic data (MK-7 as the major menaquinone and anteiso-C(15 : 0) and iso-C(16 : 0) as the predominant fatty acids) supported the affiliation of strain Gsoil 1138(T) to the genus Paenibacillus. The results of DNA-DNA hybridization experiments and physiological and biochemical tests allowed strain Gsoil 1138(T) to be distinguished genotypically and phenotypically from recognized species of the genus Paenibacillus. Strain Gsoil 1138(T) is therefore considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus pocheonensis sp. nov. is proposed. The type strain is Gsoil 1138(T) (=KCTC 13941(T)=LMG 23404(T)).
一株革兰氏阳性、需氧或兼性厌氧、杆状、产芽孢的细菌，菌株 Gsoil 1138(T)，从韩国抱川省人参田的土壤中分离出来，并对其进行了特征描述，以确定其分类地位。基于 16S rRNA 基因序列分析，菌株 Gsoil 1138(T)属于芽孢杆菌科，与粘质沙雷氏菌（98.2%相似）和解淀粉芽孢杆菌（96.5%相似）的模式菌株最为密切相关。菌株 Gsoil 1138(T)与其他公认的芽孢杆菌属种的模式菌株之间的 16S rRNA 基因序列相似性水平低于 96.5%。菌株 Gsoil 1138(T)的基因组 DNA 的 G+C 含量为 52.1+/-0.2 mol%（三次测定的平均值+/-标准差）。表型和化学分类学数据（MK-7 为主要的menaquinone，anteiso-C(15 : 0)和 iso-C(16 : 0)为主要的脂肪酸）支持菌株 Gsoil 1138(T)属于芽孢杆菌属。DNA-DNA 杂交实验和生理生化试验的结果允许菌株 Gsoil 1138(T)在基因型和表型上与公认的芽孢杆菌属种区分开来。因此，菌株 Gsoil 1138(T)被认为代表一个新的芽孢杆菌属种，建议将其命名为抱川芽孢杆菌新种。模式菌株为 Gsoil 1138(T)=KCTC 13941(T)=LMG 23404(T)。
