Hastings K L, Thomas C, Hubbard A K, Gandolfi A J
Department of Anesthesiology, University of Arizona, College of Medicine, Tucson 85724.
Br J Anaesth. 1991 Dec;67(6):722-8. doi: 10.1093/bja/67.6.722.
The diagnosis of halothane hepatitis (HH) may be assisted by detection of antibodies reacting to trifluoroacetylated proteins (anti-TFA antibodies). An enzyme-linked immunosorbent assay (ELISA) utilizing trifluoroacetylated rabbit serum albumin (TFA-RSA) as antigen detected anti-TFA antibodies in 67% of sera from patients for whom a clinical diagnosis of HH was made. Anti-TFA antibodies were detected in 33% of sera when using an ELISA with liver microsomal protein from halothane-treated rabbits as antigen. Absorption of the sera with untreated rabbit liver microsomal protein before using the microsomal protein ELISA resulted in detection of anti-TFA antibodies in 42% of sera. Using the presumptive hapten N-epsilon-trifluoroacetyl-1-lysine to block antibody binding in an ELISA resulted in positive detection in 50% of sera: the results did not always agree with the other ELISA methods. The TFA-RSA ELISA was the most sensitive method and, combined with the TFA-lysine blocking ELISA, resulted in 92% of sera from HH patients testing positive for HH-associated antibodies.
检测对三氟乙酰化蛋白产生反应的抗体（抗三氟乙酰抗体）有助于诊断氟烷性肝炎（HH）。以三氟乙酰化兔血清白蛋白（TFA-RSA）作为抗原的酶联免疫吸附测定（ELISA），在临床诊断为HH的患者的67%的血清中检测到了抗三氟乙酰抗体。当使用以经氟烷处理的兔肝脏微粒体蛋白作为抗原的ELISA时，在33%的血清中检测到了抗三氟乙酰抗体。在使用微粒体蛋白ELISA之前，用未经处理的兔肝脏微粒体蛋白吸收血清，结果在42%的血清中检测到了抗三氟乙酰抗体。在ELISA中使用推定的半抗原N-ε-三氟乙酰-1-赖氨酸来阻断抗体结合，结果在50%的血清中检测呈阳性：结果并不总是与其他ELISA方法一致。TFA-RSA ELISA是最敏感的方法，与TFA-赖氨酸阻断ELISA相结合，92%的HH患者血清检测出与HH相关抗体呈阳性。
