Callis A H, Brooks S D, Roth T P, Gandolfi A J, Brown B R
Clin Exp Immunol. 1987 Feb;67(2):343-51.
An animal model of halothane-induced liver injury has been developed in the rabbit to study the production of humoral immunity towards a biotransformation intermediate of halothane. Rabbits exposed many times to halothane in a 75% O2/25% N2 atmosphere produce an antibody that cross-reacts with the trifluoroacetyl moiety of trifluoroacetylated rabbit serum albumin (TFA-RSA). The generation of this halothane-induced immunogen is dependent upon high oxygen tension as shown by the minimal anti-TFA antibody response seen in rabbits exposed to halothane in a 14% O2/86% N2 atmosphere. In addition, halothane exposure of rabbits specifically immunized with the metabolite-carrier complex, TFA-RSA, induces a secondary antibody response toward the immunogen. In rabbits, either immunized with TFA-RSA or not, multiple halothane exposures induce populations of antibodies with varying specificities. Evidence suggests that predominance of the metabolic intermediate, the ensuing immunogen, and the subsequent antibody response depends upon the oxygen tension during successive exposures to halothane. These successive exposures could potentially generate many different immunogens resulting in varied antibody specificities.
已在兔中建立了氟烷诱导的肝损伤动物模型，以研究针对氟烷生物转化中间体的体液免疫产生情况。在75%氧气/25%氮气的环境中多次接触氟烷的兔会产生一种抗体，该抗体与三氟乙酰化兔血清白蛋白（TFA-RSA）的三氟乙酰部分发生交叉反应。如在14%氧气/86%氮气环境中接触氟烷的兔所出现的最小抗TFA抗体反应所示，这种氟烷诱导的免疫原的产生依赖于高氧张力。此外，用代谢物-载体复合物TFA-RSA进行特异性免疫的兔接触氟烷后，会诱导对免疫原的二次抗体反应。在兔中，无论是否用TFA-RSA免疫，多次接触氟烷都会诱导产生具有不同特异性的抗体群体。有证据表明，代谢中间体、随之产生的免疫原以及随后的抗体反应的优势取决于连续接触氟烷期间的氧张力。这些连续接触可能会潜在地产生许多不同的免疫原，从而导致抗体特异性各异。
