Martin J L, Kenna J G, Pohl L R
Laboratory of Chemical Pharmacology, National Heart, Lung and Blood Institute, Bethesda, Maryland 20892.
Anesth Analg. 1990 Feb;70(2):154-9. doi: 10.1213/00000539-199002000-00004.
Sera from patients with a clinical diagnosis of halothane hepatitis have been shown to contain antibodies that react with liver microsomal proteins (100, 76, 59, 57, and 54 kDa) covalently altered by the trifluoroacetyl (TFA) halide metabolite of halothane. In the present study, rapid and sensitive enzyme-linked immunosorbent assays for the detection of these antibodies have been evaluated. A recently described method that utilizes TFA-rabbit serum albumin as test antigen was studied employing a large population of halothane hepatitis and control patients. Several problems were discovered with the assay that were not previously recognized. The assay was then compared directly with methods that utilize as test antigens either liver microsomes or purified TFA proteins from halothane-treated rats. Sixty-seven percent of patients with a clinical diagnosis of halothane hepatitis tested positive for antibodies when the test antigens were either TFA-rabbit serum albumin or liver microsomes. This value was increased to 79% when the purified TFA-57 kDa, TFA-76 kDa, and TFA-100 kDa proteins were used as test antigens. These results indicate that the specificity and sensitivity of enzyme-linked immunosorbent assay methods for the detection of patients' antibodies may be increased significantly by utilizing the purified TFA microsomal proteins as test antigens.
临床诊断为氟烷性肝炎患者的血清已显示含有与经氟烷的三氟乙酰（TFA）卤化物代谢物共价修饰的肝微粒体蛋白（100、76、59、57和54 kDa）发生反应的抗体。在本研究中，对用于检测这些抗体的快速灵敏的酶联免疫吸附测定法进行了评估。利用TFA - 兔血清白蛋白作为检测抗原的一种最近描述的方法，在大量氟烷性肝炎患者和对照患者中进行了研究。该测定法发现了几个以前未被认识到的问题。然后将该测定法直接与使用肝微粒体或来自氟烷处理大鼠的纯化TFA蛋白作为检测抗原的方法进行比较。当检测抗原为TFA - 兔血清白蛋白或肝微粒体时，临床诊断为氟烷性肝炎的患者中有67%抗体检测呈阳性。当使用纯化的TFA - 57 kDa、TFA - 76 kDa和TFA - 100 kDa蛋白作为检测抗原时，该值增加到79%。这些结果表明，通过使用纯化的TFA微粒体蛋白作为检测抗原，酶联免疫吸附测定法检测患者抗体的特异性和灵敏度可能会显著提高。
