Kinel-Tahan Yael, Weiss Hanna, Dgany Orly, Levine Anna, Wides Ron
The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel.
Dev Dyn. 2007 Sep;236(9):2541-54. doi: 10.1002/dvdy.21284.
The Drosophila melanogaster pair-rule gene odz (odd Oz, or Ten-m) is expressed in distinct patterns in the larval eye imaginal disc. Its earliest eye expression occurs in ommatidial precursors starting from the posterior edge of the morphogenetic furrow. Loss of function of odz activity leads to visible light photoreceptor loss; R7 photoreceptor loss; ommatidial size, shape, and rotation defects; ommatidial disorder and fusions; interommatidial bristle defects; and ommatidial lens defects. The same effects are seen in odz eye mitotic clones, in odz-Ten-a transheterozygous combinations, and in eyes expressing an Odz-Dominant Negative transgene (Odz-DN). Effects of the same strength are also seen when the Odz-DN transgene is driven only in regions of scabrous expression, which overlaps the four columns of Odz expression clusters behind the furrow. Small odz mitotic clones suggest an odz role in cell proliferation or survival. Senseless is expressed in odz mutant clones, in a fairly ordered manner, indicating that Odz acts downstream of R8 specification. Disorder within each ommatidium in odz clones is accompanied by some loss of R7 precursors and visible photoreceptor precursor order.
果蝇的成对规则基因odz（奇数奥兹，或Ten-m）在幼虫眼成虫盘中以不同模式表达。其在眼中最早的表达出现在从形态发生沟后缘开始的小眼原基中。odz活性的功能丧失会导致可见光光感受器丧失；R7光感受器丧失；小眼大小、形状和旋转缺陷；小眼紊乱和融合；小眼间刚毛缺陷；以及小眼晶状体缺陷。在odz眼有丝分裂克隆、odz-Ten-a反式杂合组合以及表达Odz显性负性转基因（Odz-DN）的眼中也观察到了相同的效应。当Odz-DN转基因仅在粗糙表达区域驱动时，也会出现相同强度的效应，该区域与沟后Odz表达簇的四列重叠。小的odz有丝分裂克隆表明odz在细胞增殖或存活中起作用。无意义蛋白在odz突变克隆中以相当有序的方式表达，表明Odz在R8特化的下游起作用。odz克隆中小眼内的紊乱伴随着一些R7前体的丧失和可见的光感受器前体排列。
