Fan Yanru, Han Yuchun, Wang Yilin
Key Laboratory of Colloid and Interface Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, People's Republic of China.
J Phys Chem B. 2007 Aug 30;111(34):10123-9. doi: 10.1021/jp0735637. Epub 2007 Aug 9.
The interaction of hydrophobically modified copolymers of acrylamide and acrylic acid, designated as PAM-C12-AA (X%) (X% indicates the percentage of acrylic acid unit and X = 5, 10, 20), with dimyristoylphosphatidylcholine (DMPC) vesicles has been studied. Complementary techniques including isothermal titration microcalorimetry (ITC), differential scanning calorimetry (DSC), turbidity measurement, calcein leakage measurement, dynamic light scattering (DLS), and transmission electron microscopy (TEM) were used to get comprehensive information. The results show that PAM-C12-AA leads to solubilization of DMPC vesicles. There is a critical concentration (C(s)) for PAM-C12-AA to induce obvious vesicle disruption. This concentration is very close to the critical aggregation concentration (CAC) for the polymer self-aggregation. The Cs values are found to be similar for the three polymers. However, the disruption of DMPC vesicles induced by the polymers increases to a greater degree at higher AA fraction, owing to the increasing strength of interaction between the polymer and the lipid bilayer.
已对称为PAM-C12-AA（X%）（X%表示丙烯酸单元的百分比，X = 5、10、20）的丙烯酰胺与丙烯酸的疏水改性共聚物与二肉豆蔻酰磷脂酰胆碱（DMPC）囊泡之间的相互作用进行了研究。使用了包括等温滴定量热法（ITC）、差示扫描量热法（DSC）、浊度测量、钙黄绿素泄漏测量、动态光散射（DLS）和透射电子显微镜（TEM）在内的互补技术来获取全面信息。结果表明，PAM-C12-AA会导致DMPC囊泡的溶解。PAM-C12-AA诱导明显囊泡破坏存在一个临界浓度（C(s)）。该浓度非常接近聚合物自聚集的临界聚集浓度（CAC）。发现这三种聚合物的Cs值相似。然而，由于聚合物与脂质双层之间相互作用强度的增加，聚合物诱导的DMPC囊泡破坏在较高的AA分数下增加到更大程度。
