Ji Ya-Kun, Ling Jun-Qi
Department of Cariology and Endodontology, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, China.
Zhonghua Kou Qiang Yi Xue Za Zhi. 2007 May;42(5):294-7.
To examine the spatial distribution of dead and vital bacteria in the early formation of native dental biofilm.
An experimental dental biofilm model in the oral cavity was established by enamel slabs and the spatial distribution of dead and vital bacteria in the early colonization of native dental biofilm on the enamel surface was observed by in situ real-time and dynamic observations and optical sections utilizing confocal laser scanning microscope (CLSM) and live and dead bacterial fluorescence staining technique.
At the initial stage of dental biofilm formation, the structure was sparse and the percentage of dead cells reached 70% - 80% at the inner layers. In the middle layers the structure became denser than in the inner layers, which was mainly composed of vital cells (40% - 70%), and void-like structures were surrounded by vital bacteria. In the outer layers, the structure was sparse and vital cells occupied 20% - 40%.
Native dental biofilms showed an uneven spatial distribution of vital and dead microorganisms. The percentage of vital microorganisms was lower adjacent to the enamel surface, increased in the z-axis towards the central parts, and decreased again towards the outer layers. The dead bacteria is an integral component in the early formation of native dental biofilm. Bacteria in the biofilms increased with time forming abundant channels.
研究天然牙菌斑早期形成过程中死菌和活菌的空间分布情况。
采用釉质块在口腔内建立实验性牙菌斑模型，利用共聚焦激光扫描显微镜（CLSM）和死活细菌荧光染色技术，通过原位实时动态观察和光学切片，观察天然牙菌斑在釉质表面早期定植过程中死菌和活菌的空间分布。
在牙菌斑形成初期，结构稀疏，内层死细胞百分比达70% - 80%。中层结构比内层致密，主要由活菌组成（40% - 70%），类空结构被活菌包围。外层结构稀疏，活菌占20% - 40%。
天然牙菌斑中活菌和死菌的空间分布不均匀。靠近釉质表面的活菌百分比较低，在z轴方向朝中心部分增加，然后朝外层又降低。死菌是天然牙菌斑早期形成的一个组成部分。牙菌斑中的细菌随时间增加，形成丰富的通道。
