State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
College of Polymer Science and Engineering, Sichuan University, Chengdu, China.
Int J Oral Sci. 2020 Sep 29;12(1):27. doi: 10.1038/s41368-020-00097-y.
Tooth enamel is prone to be attacked by injurious factors, leading to a de/remineralization imbalance. To repair demineralized enamel and prevent pulp inflammation caused by biofilm accumulation, measures are needed to promote remineralization and inhibit bacterial adhesion on the tooth surface. An innovative material, poly (aspartic acid)-polyethylene glycol (PASP-PEG), was designed and synthesized to construct a mineralizing and anti-adhesive surface that could be applied to repair demineralized enamel. A cytotoxicity assay revealed the low cytotoxicity of synthesized PASP-PEG. Adsorption results demonstrated that PASP-PEG possesses a high binding affinity to the hydroxyapatite (HA)/tooth surface. In vitro experiments and scanning electron microscopy (SEM) demonstrated a strong capacity of PASP-PEG to induce in situ remineralization and direct the oriented growth of apatite nanocrystals. Energy dispersive X-ray spectroscopy (EDS), X-ray diffraction analysis (XRD) and Vickers hardness tests demonstrated that minerals induced by PASP-PEG were consistent with healthy enamel in Ca/P ratio, crystal form and surface micro-hardness. Contact angle tests and bacterial adhesion experiments demonstrated that PASP-PEG yielded a strong anti-adhesive effect. In summary, PASP-PEG could achieve dual effects for enamel repair and anti-adhesion of bacteria, thereby widening its application in enamel repair.
牙釉质容易受到有害因素的攻击，导致脱矿/再矿化失衡。为了修复脱矿的牙釉质并防止生物膜积聚引起的牙髓炎症，需要采取措施促进再矿化并抑制细菌在牙齿表面的黏附。一种创新材料聚（天冬氨酸）-聚乙二醇（PASP-PEG）被设计并合成，以构建一种矿化和抗黏附表面，可用于修复脱矿的牙釉质。细胞毒性试验表明合成的 PASP-PEG 具有低细胞毒性。吸附结果表明，PASP-PEG 对羟基磷灰石（HA）/牙齿表面具有高结合亲和力。体外实验和扫描电子显微镜（SEM）表明，PASP-PEG 具有很强的诱导原位再矿化和引导磷灰石纳米晶体定向生长的能力。能量色散 X 射线光谱（EDS）、X 射线衍射分析（XRD）和维氏硬度测试表明，PASP-PEG 诱导的矿物质在 Ca/P 比、晶体形态和表面微观硬度方面与健康牙釉质一致。接触角测试和细菌黏附实验表明，PASP-PEG 具有很强的抗黏附作用。总之，PASP-PEG 可以实现牙釉质修复和细菌抗黏附的双重效果，从而拓宽了其在牙釉质修复中的应用。
