Yang X L, Huang C, Qiao X G, Yao L, Zhao D X, Tan X
School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, China.
Toxicol In Vitro. 2007 Dec;21(8):1493-8. doi: 10.1016/j.tiv.2007.06.008. Epub 2007 Jun 30.
Using human erythrocyte membranes (EMs) as a model system, we have examined photo-induced lipid peroxidation by a bis-methanophosphonate fullerene (BMPF) and four other fullerene derivatives including a mono-methanophosphonic acid fullerene (MMPF), a dimalonic acid C(60) (DMA C(60)), a trimalonic acid C(60) (TMA C(60)) and a polyhydroxylated fullerene (fullerol). Lipid peroxidation was assessed as the malondialdehyde (MDA) level measured by the thiobarbituric acid assay. It was observed that BMPF increased the MDA level of EMs after irradiation in both time- and dose-dependent manners. The photo-induced activity became very significant (p<0.01) under the conditions of either the concentration of 10 microM and irradiation time of 30 min or the concentration of 5 microM and irradiation time of 60 min. Involvement of reactive oxygen species (ROS) in the activity was also examined by specific inhibitors of singlet oxygen, superoxide anions and hydroxyl radicals, respectively. While all three kinds were found responsible for the activity, the former two might play more important roles than the last one. Furthermore, the activity of BMPF was the strongest among all tested fullerene derivatives. These results indicated BMPF was a potential photosensitizer that would find application in photodynamic therapy.
以人红细胞膜（EMs）作为模型系统，我们研究了双甲膦酸富勒烯（BMPF）以及其他四种富勒烯衍生物（包括单甲膦酸富勒烯（MMPF）、二丙二酸C60（DMA C60）、三丙二酸C60（TMA C60）和多羟基化富勒烯（富勒醇））的光诱导脂质过氧化作用。脂质过氧化作用通过硫代巴比妥酸法测定的丙二醛（MDA）水平来评估。结果发现，照射后BMPF能以时间和剂量依赖性方式提高EMs的MDA水平。在浓度为10 microM、照射时间为30分钟或浓度为5 microM、照射时间为60分钟的条件下，光诱导活性变得非常显著（p<0.01）。还分别通过单线态氧、超氧阴离子和羟基自由基的特异性抑制剂研究了活性氧（ROS）在该活性中的作用。虽然发现这三种物质都与该活性有关，但前两者可能比后者发挥更重要的作用。此外，在所有测试的富勒烯衍生物中，BMPF的活性最强。这些结果表明BMPF是一种潜在的光敏剂，可应用于光动力疗法。
