School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, PR China.
J Photochem Photobiol B. 2010 Mar 8;98(3):193-8. doi: 10.1016/j.jphotobiol.2010.01.001. Epub 2010 Jan 13.
Photo-excited bioactivities of fullerene derivatives are attracting much attention. In this report, a bis-methanophosphonate fullerene (BMPF) and the other two fullerene derivatives, a bis-malonic acid fullerene (BMAF) and a fullerol were incubated with HeLa cells and irradiated with a green light emitted from a mercury lamp on a fluorescent microscopy. By using DNA fluorescent probe propidium iodide staining method, damage towards cell membrane could be detected when cells were treated by irradiation altogether with BMPF or BMAF at a low concentration (4 microM), and the damage was dose-dependent. The activity of BMPF was much higher than that of BMAF, while fullerol had no effects under the same condition. It was also revealed that different kinds of reactive oxygen species (ROS) correlated to BMPF and BMAF. Additionally, presence of extracellular calcium could promote the activities of both derivatives, while removal of extracellular calcium could not abort their membrane-damaged activities. These results indicated that ROS and calcium were involved in the photosensitization of fullerene derivatives, and BMPF was a superior photosensitizer which would find potential application in biomedical field.
富勒烯衍生物的光激发生物活性引起了广泛关注。在本报告中，我们将二甲基膦酸酯富勒烯（BMPF）与另外两种富勒烯衍生物，即双丙二酸富勒烯（BMAF）和富勒醇一起孵育 HeLa 细胞，并通过汞灯发出的绿光在荧光显微镜下进行照射。通过使用 DNA 荧光探针碘化丙啶染色方法，当用 BMPF 或 BMAF（4μM）低浓度照射细胞时，可检测到细胞膜损伤，并且损伤呈剂量依赖性。BMPF 的活性远高于 BMAF，而在相同条件下富勒醇则没有影响。此外，研究还揭示了与 BMPF 和 BMAF 相关的不同种类的活性氧（ROS）。此外，细胞外钙的存在可以促进两种衍生物的活性，而去除细胞外钙并不能终止它们的膜损伤活性。这些结果表明，ROS 和钙参与了富勒烯衍生物的光致敏作用，BMPF 是一种优越的光敏剂，将在生物医学领域有潜在的应用前景。
