Sakagami H, Kikuchi K, Takeda M, Sato T, Ichikawa S, Fujimaki M, Wada C, Komatsu N
First Department of Biochemistry, School of Medicine, Showa University, Tokyo, Japan.
In Vivo. 1991 Mar-Apr;5(2):101-5.
Pretreatment with N,N-dimethylaminoethyl (DMAE) paramylon significantly protected mice from infection by various microorganisms. When mouse peritoneal macrophages were cultured with DMAE-paramylon, they showed morphological change (spreading) and elevated NBT-reducing activity. Macrophages prepared from DMAE-paramylon-treated mice had higher NBT-reducing activity than those from control mice. On the other hand, carboxymethyl paramylon and paramylon sulfate, which did not induce appreciable antimicrobial activity, failed to stimulate macrophage functions. The data suggest a significant role of macrophages in antimicrobial activity induction by cationized glucans.
用N，N-二甲基氨基乙基（DMAE）-副淀粉预处理可显著保护小鼠免受多种微生物感染。当小鼠腹腔巨噬细胞与DMAE-副淀粉一起培养时，它们表现出形态变化（铺展）和NBT还原活性升高。从经DMAE-副淀粉处理的小鼠制备的巨噬细胞比对照小鼠的巨噬细胞具有更高的NBT还原活性。另一方面，未诱导出明显抗菌活性的羧甲基副淀粉和硫酸副淀粉未能刺激巨噬细胞功能。这些数据表明巨噬细胞在阳离子化葡聚糖诱导抗菌活性中起重要作用。
