Kierska D, Maśliński C
Agents Actions. 1975 Dec;5(5):471-5. doi: 10.1007/BF01972683.
The dynamics of the complex formation between pyridoxal 5'-phosphate (PLP) and histidine in the presence of bacterial histidine decarboxylase was examined. Since PLP is able to form a cyclic product with histidine and histamine, the possibility of complex formation between PLP and histamine formed during a decarboxylation reaction was examined too. It was found that the cyclization reaction between PLP and histidine is equimolecular and the rate of cyclic product formation is not significantly influenced by the presence of enzyme. In the presence of bacterial histidine decarboxylase both the cyclization reaction and cyclic product formation were observed. Predominance of histamine or cyclic product formation was dependent on pH and substrate concentration. In the presence of histidine and enzymatic protein, histamine formed during the decarboxylation reaction was unable to form a cyclic product with PLP.
研究了在细菌组氨酸脱羧酶存在下，磷酸吡哆醛（PLP）与组氨酸形成复合物的动力学。由于PLP能够与组氨酸和组胺形成环状产物，因此也研究了在脱羧反应过程中形成的PLP与组胺之间形成复合物的可能性。发现PLP与组氨酸之间的环化反应是等分子的，并且环状产物的形成速率不受酶的存在的显著影响。在细菌组氨酸脱羧酶存在下，观察到环化反应和环状产物的形成。组胺或环状产物形成的优势取决于pH值和底物浓度。在组氨酸和酶蛋白存在下，脱羧反应过程中形成的组胺无法与PLP形成环状产物。
