Thian E S, Huang J, Ahmad Z, Edirisinghe M J, Jayasinghe S N, Ireland D C, Brooks R A, Rushton N, Best S M, Bonfield W
Department of Materials Science and Metallurgy, University of Cambridge, Pembroke Street, Cambridge CB2 3QZ, UK.
J Biomed Mater Res A. 2008 Apr;85(1):188-94. doi: 10.1002/jbm.a.31564.
Electrohydrodynamic spraying has been used to produce patterns of line width up to 100 microm in size on glass discs, using nanohydroxyapatite (nHA). A human osteoblast (HOB)-like cell model was then used to study the interaction between the HOB cells and nHA patterns in vitro. Growth of the cells was significantly increased (p < 0.05) on the nHA surfaces. In addition, HOBs attached and spread well, secreting extracellular matrix. It was found that a confluent, aligned cell layer was achieved on nHA patterns by day 9. Immunofluorescent staining indicated that these cells showed elongated nuclei, enhanced adhesion (vinculin adhesion plaques) and a well-aligned cytoskeleton (actin stress fibres). This work suggests that this type of spraying may provide a route for the production of nanoscale features on implants for biomedical applications.
利用纳米羟基磷灰石（nHA），通过电液动力喷涂在玻璃圆盘上制备了尺寸达100微米的线宽图案。随后使用类人成骨细胞（HOB）模型在体外研究HOB细胞与nHA图案之间的相互作用。在nHA表面，细胞生长显著增加（p < 0.05）。此外，HOB细胞附着良好且铺展良好，并分泌细胞外基质。结果发现，到第9天时，在nHA图案上形成了汇合且排列整齐的细胞层。免疫荧光染色表明，这些细胞呈现出细长的细胞核、增强的黏附（黏着斑）以及排列良好的细胞骨架（肌动蛋白应力纤维）。这项工作表明，这种喷涂方式可能为生物医学应用的植入物上制备纳米级特征提供一条途径。
