Lu Ming-Chin, Hsiang Shih-Wei, Lai Tung-Yuan, Yao Chun-Hsu, Lin Li-Yu, Chen Yueh-Sheng
School of Post-Baccalaureat Chinese Medicine, China Medical University, Taichung, Taiwan.
J Biomater Sci Polym Ed. 2007;18(7):843-63. doi: 10.1163/156856207781367747.
We evaluated peripheral nerve regeneration using biodegradable genipin-cross-linked gelatin nerve conduits (GGCs) with three different cross-linking degrees, 24, 36 and 51%. Biocompatibility and biodegradability of the GGC and its efficiency as a guidance channel were examined based on the repair process of a 10-mm gap in the rat sciatic nerve. From this pilot study we concluded that GGCs with a mean cross-linking degree of 36% can ensure nerve regeneration with a more mature structure, as demonstrated by better developed epineural and perineural organisation and axonal development, as well as better-recovered electrophysiology with a relatively positive sciatic functional index and a shorter latency of the muscle action potential curve. Regenerated nerves in the GGCs with mean cross-linking degrees of 24 and 51% were less favourable, due to irritation caused by degradation material and compression by the remaining tube walls, respectively.
我们使用具有24%、36%和51%三种不同交联度的可生物降解的京尼平交联明胶神经导管（GGCs）评估周围神经再生。基于大鼠坐骨神经10毫米间隙的修复过程，研究了GGC的生物相容性和生物降解性及其作为引导通道的效率。从这项初步研究中我们得出结论，平均交联度为36%的GGCs能够确保神经再生，其结构更成熟，表现为神经外膜和神经束膜组织以及轴突发育更好，并且电生理恢复更好，坐骨神经功能指数相对为正，肌肉动作电位曲线潜伏期更短。平均交联度为24%和51%的GGCs中的再生神经则不太理想，分别是由于降解材料引起的刺激和剩余管壁的压迫。
