Krikorian R, Lozach-Perlant A, Ferrier-Rembert A, Hoerner P, Sonntag P, Garin D, Crance J-M
Hutchinson Santé, Liancourt, France.
J Hosp Infect. 2007 Aug;66(4):339-45. doi: 10.1016/j.jhin.2007.05.008. Epub 2007 Aug 6.
Rubber surgical gloves worn as a barrier to prevent contamination from body fluids offer relative protection against contamination through direct percutaneous injuries involving needles, scalpel blades or bone fragments. To determine the main experimental parameters influencing the volume of blood transmitted by a hollow-bore needle (worst case scenario) during an accidental puncture, we designed an automatic puncture apparatus. Herpes simplex type 1 virus (HSV1), a model for enveloped viruses, was used as a 'marker' in an in-vitro gelatine model. Of the experimental parameters studied, the most critical influences were found to be needle diameter and puncture depth, whereas puncture speed, puncture angle and glove-stretching feature appeared to be less influential. A single glove reduced the volume of blood transferred by 52% compared with no glove, but double gloving offered no additional protection against hollow-bore needle punctures. Using 'standardized' puncture conditions, the virus-inhibiting surgical glove G-VIR elicited an 81% reduction in the amount of HSV1 transmitted as compared with single or double latex glove systems.
作为防止体液污染屏障的橡胶手术手套，能为涉及针头、手术刀刀片或骨碎片的直接经皮损伤提供相对的防污染保护。为了确定意外穿刺期间影响空心针（最坏情况）传输血液量的主要实验参数，我们设计了一种自动穿刺装置。单纯疱疹病毒1型（HSV1）作为包膜病毒的模型，被用作体外明胶模型中的“标记物”。在所研究的实验参数中，发现最关键的影响因素是针的直径和穿刺深度，而穿刺速度、穿刺角度和手套拉伸特性的影响似乎较小。与不戴手套相比，单层手套可使传输的血液量减少52%，但双层手套对空心针穿刺并无额外保护作用。在“标准化”穿刺条件下，与单层或双层乳胶手套系统相比，具有病毒抑制功能的手术手套G-VIR可使HSV1的传输量减少81%。
