Bennett N T, Howard R J
Department of Surgery, University of Florida, Gainesville 32610.
J Am Coll Surg. 1994 Feb;178(2):107-10.
We wanted to quantify the amount of blood inoculum present on several commonly used surgical and phlebotomy needles and to determine the effect of single or double gloving, the depth of needle penetration and needle size on inoculum volume. Nineteen, 22 gauge and 25 gauge phlebotomy needles, as well as the tapered suture needles from zero, 3-0 and 5-0 sutures and a cutting needle from a 4-0 suture were assessed. The needles were dipped into blood labeled with 125I-epidermal growth factor and then embedded 2 or 5 millimeters into an agarose gel. The volume of blood inoculum ranged from 133 to 683 nanoliters for the phlebotomy needles and from 35 to 366 nanoliters for the suture needles. Needles were then passed through none, one or two layers of surgical glove material before embedding the needles 5 millimeters into agarose gel. The use of one layer of surgical glove resulted in a significant (p < 0.001) decrease in inoculum from tapered suture needles, but not from the cutting needles. Two gloves were even more efficient (p < 0.001) than one glove at removing blood from all suture needles, including the cutting needle. However, surgical glove material did not significantly reduce the volume of blood inoculum transferred by the phlebotomy needles. The size of the needle and depth of penetration were found to have a significant influence on inoculum.
我们想要量化几种常用手术针和静脉穿刺针上存在的血液接种量，并确定单层或双层手套、针刺深度和针的尺寸对接种量的影响。评估了19号、22号和25号静脉穿刺针，以及0号、3-0号和5-0号缝线的锥形缝合针和4-0号缝线的切割针。将针浸入用125I-表皮生长因子标记的血液中，然后将其嵌入琼脂糖凝胶2或5毫米深处。静脉穿刺针的血液接种量在133至683纳升之间，缝合针的接种量在35至366纳升之间。然后，在将针嵌入琼脂糖凝胶5毫米深处之前，将针穿过零层、一层或两层手术手套材料。使用一层手术手套可使锥形缝合针的接种量显著减少（p<0.001），但切割针的接种量没有减少。两层手套在去除所有缝合针（包括切割针）上的血液方面比一层手套更有效（p<0.001）。然而，手术手套材料并没有显著减少静脉穿刺针转移的血液接种量。发现针的尺寸和穿刺深度对接种量有显著影响。
