Uyanoglu Mustafa, Canbek Mediha, Aral Erinc, Husnu Can Baser K
Faculty of Science, Department of Biology, Eskisehir Osmangazi University, 26480, Eskisehir, Turkey.
Phytomedicine. 2008 Mar;15(3):226-9. doi: 10.1016/j.phymed.2007.06.010. Epub 2007 Aug 3.
The present study aims to investigate the possible effects of carvacrol obtained from origanum oil upon the regenerative feature of the liver subsequent to partial hepatectomy in rats. Male Wistar Albino rats, weighing 230+/-30g, were divided into three experiment groups. Group I (n=8) were used as sham operation group. Group II (n=8) were applied saline solution and hepatectomy. Carvacrol and hepatectomy (73mg/kg) were applied to Group III (n=8). One dose of test material was injected 1h before 68% partial hepatectomy. At the end of the experiments, blood and organs were removed. The liver regeneration rate of the rats was calculated measuring the weights of their liver before and after the hepatectomy. Hematoxylin and eosin, interleukin-6 (IL-6) and proliferating cell nuclear antigen (PCNA) treatments were applied to liver sections. Aspartate transaminase (AST), alanine transaminase (ALT), tumor necrosis factor-alpha (TNF-alpha) and IL-6 levels were determined in serum samples. The liver regeneration, mitotic index and PCNA index increased significantly in rats of Group III over the Group II at the 72nd hour after partial hepatectomy. Histological evaluations were also similar with these results of PCNA and mitotic indexes. In AST, ALT, TNF-alpha and IL-6 levels, there was no statistically significant difference. According to these results, it is concluded that carvacrol increases the liver regeneration rate.
本研究旨在探讨牛至油中提取的香芹酚对大鼠部分肝切除术后肝脏再生特性的可能影响。将体重230±30g的雄性Wistar白化大鼠分为三个实验组。第一组（n = 8）作为假手术组。第二组（n = 8）进行生理盐水注射及肝切除术。第三组（n = 8）进行香芹酚注射（73mg/kg）及肝切除术。在68%部分肝切除术前1小时注射一剂受试材料。实验结束时，取出血液和器官。通过测量肝切除前后大鼠肝脏的重量来计算肝脏再生率。对肝脏切片进行苏木精和伊红染色、白细胞介素-6（IL-6）和增殖细胞核抗原（PCNA）处理。测定血清样本中的天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、肿瘤坏死因子-α（TNF-α）和IL-6水平。部分肝切除术后72小时，第三组大鼠的肝脏再生、有丝分裂指数和PCNA指数显著高于第二组。组织学评估结果与PCNA和有丝分裂指数的结果相似。在AST、ALT、TNF-α和IL-6水平上，无统计学显著差异。根据这些结果，得出香芹酚可提高肝脏再生率的结论。
