Canbek Mediha, Uyanoglu Mustafa, Canbek Selcuk, Ceyhan Emre, Ozen Ahmet, Durmus Basak, Turgak Ozge
Department of Biology, Faculty of Science, Eskisehir Osmangazi University, Eskisehir, Turkey
Department of Biology, Faculty of Science, Eskisehir Osmangazi University, Eskisehir, Turkey.
In Vivo. 2017 Mar-Apr;31(2):209-213. doi: 10.21873/invivo.11047.
Geraniol is a monoterpenoid alcohol that has a hepatoprotective effect. We investigated the regenerative effects of geraniol in rats after a 70% partial hepatectomy (PH). Using Wistar albino rats, nine groups were created: Group I was the control group, while the remaining groups received a single intraperitoneal dose of saline, Silymarin, or geraniol after PH. A 70% PH was performed on all groups except for groups II and III. Blood serum samples were obtained for alanine amino transferase (ALT) analysis. Then liver tissues were harvested for histological and real-time polymerase chain reaction (PCR) analyses. Tumor necrosis factor-α (TNFα) and interleukin 6 (IL6) gene expression were examined 24 and 48 h after PH. ALT levels were found to be statistically significantly increased in all PH-treated groups. TNFα and IL6 gene expression levels were elevated in geraniol-treated groups. Histological evaluation revealed a hepatoprotective effect for geraniol-treated groups. Our results suggest that geraniol plays a significant role during liver regeneration, which involves the elevated expression of TNFα and IL6 48 h after PH.
香叶醇是一种具有肝脏保护作用的单萜醇。我们研究了香叶醇对70%部分肝切除（PH）大鼠的再生作用。使用Wistar白化大鼠，分为九组：第一组为对照组，其余组在PH后腹腔注射单次剂量的生理盐水、水飞蓟宾或香叶醇。除第二组和第三组外，所有组均进行70%的PH。采集血清样本进行丙氨酸氨基转移酶（ALT）分析。然后收获肝脏组织进行组织学和实时聚合酶链反应（PCR）分析。在PH后24小时和48小时检测肿瘤坏死因子-α（TNFα）和白细胞介素6（IL6）基因表达。发现所有PH治疗组的ALT水平在统计学上显著升高。香叶醇治疗组的TNFα和IL6基因表达水平升高。组织学评估显示香叶醇治疗组具有肝脏保护作用。我们的结果表明，香叶醇在肝脏再生过程中起重要作用，这涉及到PH后48小时TNFα和IL6表达的升高。
