Boer Klas, Brehmer-Streck Sybille, Deufel Thomas, Schmidt Dirk, Kiehntopf Michael
Institut fuer Klinische Chemie und Labordiagnostik FSU, Jena, Germany.
Clin Biochem. 2007 Oct;40(15):1163-7. doi: 10.1016/j.clinbiochem.2007.06.011. Epub 2007 Jul 5.
Evaluation of the performance of the EMIT 2000 Cyclosporin assay using 2 sets of assay calibrators and the EMIT 2000 mycophenolic acid assay to measure C0 and C2 concentrations on the Abbott Architect c8000 analyzer.
Imprecision studies were performed. Cyclosporin concentration was assayed by EMIT on the c8000, by ACMIA on the Dimension and by LC-MS/MS while mycophenolic acid was analyzed by EMIT on c8000 and on Dimension and by HPLC.
Agreement between cyclosporin and mycophenolic acid concentrations assayed on the c8000 and on the Dimension was very good. Method comparison between the c8000 and LC-MS/MS resulted in a relative bias of 15.7% for C0 and 11.5% for C2 concentrations. Relative bias of the mycophenolic acid concentrations assayed on the c8000 and the HPLC was 37.7%.
When reported properly to the clinician mycophenolic acid and cyclosporine blood levels can be monitored using the EMIT assays on the c8000 consolidating standard routine workflow and reducing reagent costs significantly.
使用两组分析校准品评估EMIT 2000环孢素检测方法的性能，并使用EMIT 2000霉酚酸检测方法在雅培Architect c8000分析仪上测量C0和C2浓度。
进行不精密度研究。环孢素浓度通过c8000上的EMIT、Dimension上的ACMIA以及液相色谱-串联质谱法（LC-MS/MS）进行检测，而霉酚酸则通过c8000和Dimension上的EMIT以及高效液相色谱法（HPLC）进行分析。
在c8000和Dimension上检测的环孢素和霉酚酸浓度之间的一致性非常好。c8000与LC-MS/MS之间的方法比较得出，C0浓度的相对偏差为15.7%，C2浓度的相对偏差为11.5%。在c8000上检测的霉酚酸浓度与HPLC之间的相对偏差为37.7%。
当向临床医生正确报告时，可使用c8000上的EMIT检测方法监测霉酚酸和环孢素的血药浓度，整合标准常规工作流程并显著降低试剂成本。
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