嗜温嗜水气单胞菌菌株的完整III型分泌系统
Complete type III secretion system of a mesophilic Aeromonas hydrophila strain.
作者信息
Vilches Silvia, Urgell Cecilia, Merino Susana, Chacón Matilde R, Soler Lara, Castro-Escarpulli Graciela, Figueras Maria Jose, Tomás Juan M
机构信息
Departamento Microbiología, Facultad Biología, Universidad de Barcelona, Barcelona, Spain.
出版信息
Appl Environ Microbiol. 2004 Nov;70(11):6914-9. doi: 10.1128/AEM.70.11.6914-6919.2004.
Abstract
We have investigated the existence and genetic organization of a functional type III secretion system (TTSS) in a mesophilic Aeromonas strain by initially using the Aeromonas hydrophila strain AH-3. We report for the first time the complete TTSS DNA sequence of an Aeromonas strain that comprises 35 genes organized in a similar disposition as that in Pseudomonas aeruginosa. Using several gene probes, we also determined the presence of a TTSS in clinical or environmental strains of different Aeromonas species: A. hydrophila, A. veronii, and A. caviae. By using one of the TTSS genes (ascV), we were able to obtain a defined insertion mutant in strain AH-3 (AH-3AscV), which showed reduced toxicity and virulence in comparison with the wild-type strain. Complementation of the mutant strain with a plasmid vector carrying ascV was fully able to restore the wild-type toxicity and virulence.
摘要
我们最初使用嗜水气单胞菌菌株AH-3，研究了嗜温气单胞菌菌株中功能性III型分泌系统（TTSS）的存在及其基因组织。我们首次报告了气单胞菌菌株完整的TTSS DNA序列，该序列由35个基因组成，其排列方式与铜绿假单胞菌中的相似。使用几种基因探针，我们还确定了不同气单胞菌物种的临床或环境菌株中存在TTSS，这些物种包括嗜水气单胞菌、维罗纳气单胞菌和豚鼠气单胞菌。通过使用TTSS基因之一（ascV），我们在菌株AH-3中获得了一个明确的插入突变体（AH-3AscV），与野生型菌株相比，该突变体的毒性和毒力有所降低。用携带ascV的质粒载体对突变菌株进行互补，完全能够恢复野生型的毒性和毒力。
相似文献
1
Complete type III secretion system of a mesophilic Aeromonas hydrophila strain.嗜温嗜水气单胞菌菌株的完整III型分泌系统
Appl Environ Microbiol. 2004 Nov;70(11):6914-9. doi: 10.1128/AEM.70.11.6914-6919.2004.
2
Aeromonas hydrophila AH-3 AexT is an ADP-ribosylating toxin secreted through the type III secretion system.嗜水气单胞菌AH-3 AexT是一种通过III型分泌系统分泌的ADP核糖基化毒素。
Microb Pathog. 2008 Jan;44(1):1-12. doi: 10.1016/j.micpath.2007.06.004. Epub 2007 Jul 1.
3
Identification and characterization of putative virulence genes and gene clusters in Aeromonas hydrophila PPD134/91.嗜水气单胞菌PPD134/91中假定毒力基因及基因簇的鉴定与特征分析
Appl Environ Microbiol. 2005 Aug;71(8):4469-77. doi: 10.1128/AEM.71.8.4469-4477.2005.
4
Detection of type III secretion system genes in Aeromonas hydrophila and their relationship with virulence in Nile tilapia.检测嗜水气单胞菌中的 III 型分泌系统基因及其与尼罗罗非鱼致病性的关系。
Vet Microbiol. 2010 Aug 26;144(3-4):371-6. doi: 10.1016/j.vetmic.2010.01.021. Epub 2010 Feb 1.
5
Further characterization of a type III secretion system (T3SS) and of a new effector protein from a clinical isolate of Aeromonas hydrophila--part I.嗜水气单胞菌临床分离株III型分泌系统（T3SS）及一种新效应蛋白的进一步特性分析——第一部分
Microb Pathog. 2007 Oct;43(4):127-46. doi: 10.1016/j.micpath.2007.05.002. Epub 2007 May 18.
6
The UDP N-acetylgalactosamine 4-epimerase gene is essential for mesophilic Aeromonas hydrophila serotype O34 virulence.UDP-N-乙酰半乳糖胺4-表异构酶基因对嗜温性嗜水气单胞菌O34血清型的毒力至关重要。
Infect Immun. 2006 Jan;74(1):537-48. doi: 10.1128/IAI.74.1.537-548.2006.
7
Mutation in the S-ribosylhomocysteinase (luxS) gene involved in quorum sensing affects biofilm formation and virulence in a clinical isolate of Aeromonas hydrophila.参与群体感应的S-核糖基高丝氨酸酶（luxS）基因突变影响嗜水气单胞菌临床分离株的生物膜形成和毒力。
Microb Pathog. 2008 Nov-Dec;45(5-6):343-54. doi: 10.1016/j.micpath.2008.08.007. Epub 2008 Sep 26.
8
Characterization of Aeromonas hydrophila wound pathotypes by comparative genomic and functional analyses of virulence genes.比较基因组学和毒力基因功能分析对嗜水气单胞菌创伤病原型的特征分析。
mBio. 2013 Apr 23;4(2):e00064-13. doi: 10.1128/mBio.00064-13.
9
An Aeromonas salmonicida type IV pilin is required for virulence in rainbow trout Oncorhynchus mykiss.杀鲑气单胞菌IV型菌毛蛋白是虹鳟鱼（Oncorhynchus mykiss）致病所必需的。
Dis Aquat Organ. 2002 Aug 15;51(1):13-25. doi: 10.3354/dao051013.
10
Global transcriptional responses of wild-type Aeromonas hydrophila and its virulence-deficient mutant in a murine model of infection.野生型嗜水气单胞菌及其毒力缺陷型突变体在小鼠感染模型中的全局转录反应。
Microb Pathog. 2007 May-Jun;42(5-6):193-203. doi: 10.1016/j.micpath.2007.02.002. Epub 2007 Feb 14.
引用本文的文献
1
Cytotoxicity induced by Aeromonas schubertii is orchestrated by a unique set of type III secretion system effectors.舒伯特气单胞菌诱导的细胞毒性是由一组独特的III型分泌系统效应蛋白精心调控的。
Vet Res. 2025 Jun 8;56(1):113. doi: 10.1186/s13567-025-01548-2.
2
Genomic analysis of clinical isolates reveals genetic diversity but little evidence of genetic determinants for diarrhoeal disease.临床分离株的基因组分析揭示了遗传多样性，但几乎没有发现腹泻病的遗传决定因素。
Microb Genom. 2024 Mar;10(3). doi: 10.1099/mgen.0.001211.
3
Whole genome sequence analysis of spp. isolated from ready-to-eat seafood: antimicrobial resistance and virulence factors.从即食海鲜中分离出的[物种名称]的全基因组序列分析：抗菌抗性和毒力因子。 （注：原文中“ spp.”应替换为具体物种名称，这里按原样翻译是因为不清楚具体所指物种）
Front Microbiol. 2023 Jun 30;14:1175304. doi: 10.3389/fmicb.2023.1175304. eCollection 2023.
4
Prevalence and genetic diversity of isolated from aquaculture systems in the Poyang Lake area, China.中国鄱阳湖地区水产养殖系统中分离出的[具体内容缺失]的流行情况和遗传多样性。
Front Microbiol. 2022 Dec 12;13:1042007. doi: 10.3389/fmicb.2022.1042007. eCollection 2022.
5
Potential Pathogenicity of spp. Recovered in River Water, Soil, and Vegetation from a Natural Recreational Area.从自然休闲区的河水、土壤和植被中分离出的[具体物种名称未给出]的潜在致病性
Pathogens. 2022 Nov 19;11(11):1382. doi: 10.3390/pathogens11111382.
6
PopB-PcrV Interactions Are Essential for Pore Formation in the Pseudomonas aeruginosa Type III Secretion System Translocon.PopB-PcrV 相互作用对铜绿假单胞菌 III 型分泌系统转位通道孔形成是必需的。
mBio. 2022 Oct 26;13(5):e0238122. doi: 10.1128/mbio.02381-22. Epub 2022 Sep 26.
7
Ethnobotanical study of Hakka traditional medicine in Ganzhou, China and their antibacterial, antifungal, and cytotoxic assessments.中国赣州客家传统医药的民族植物学研究及其抗菌、抗真菌和细胞毒性评估。
BMC Complement Med Ther. 2022 Sep 19;22(1):244. doi: 10.1186/s12906-022-03712-z.
8
Secretion Systems in Gram-Negative Bacterial Fish Pathogens.革兰氏阴性细菌性鱼类病原体中的分泌系统。
Front Microbiol. 2021 Dec 15;12:782673. doi: 10.3389/fmicb.2021.782673. eCollection 2021.
9
Delivering the pain: an overview of the type III secretion system with special consideration for aquatic pathogens.输送疼痛：III 型分泌系统概述，特别考虑水生病原体。
Vet Res. 2021 Dec 19;52(1):146. doi: 10.1186/s13567-021-01015-8.
10
Pancreaticobiliary Cancers and Aeromonas Isolates Carrying Type Ⅲ Secretion System Genes Are Associated With Increased Mortality: An Analysis of 164 Aeromonas Infection Episodes in Southern Taiwan.胰胆癌症与携带 III 型分泌系统基因的气单胞菌分离株与死亡率增加相关：对台湾南部 164 例气单胞菌感染病例的分析。
Front Cell Infect Microbiol. 2021 Oct 19;11:749269. doi: 10.3389/fcimb.2021.749269. eCollection 2021.
本文引用的文献
1
Type III secretion system genes in clinical Aeromonas isolates.临床气单胞菌分离株中的III型分泌系统基因。
J Clin Microbiol. 2004 Mar;42(3):1285-7. doi: 10.1128/JCM.42.3.1285-1287.2004.
2
A type III secretion system is required for Aeromonas hydrophila AH-1 pathogenesis.嗜水气单胞菌AH-1的致病机制需要III型分泌系统。
Infect Immun. 2004 Mar;72(3):1248-56. doi: 10.1128/IAI.72.3.1248-1256.2004.
3
The Klebsiella pneumoniae wabG gene: role in biosynthesis of the core lipopolysaccharide and virulence.肺炎克雷伯菌wabG基因：在核心脂多糖生物合成及毒力中的作用
J Bacteriol. 2003 Dec;185(24):7213-21. doi: 10.1128/JB.185.24.7213-7221.2003.
4
The ADP-ribosylating toxin, AexT, from Aeromonas salmonicida subsp. salmonicida is translocated via a type III secretion pathway.来自杀鲑气单胞菌杀鲑亚种的ADP核糖基化毒素AexT通过III型分泌途径转运。
J Bacteriol. 2003 Nov;185(22):6583-91. doi: 10.1128/JB.185.22.6583-6591.2003.
5
Type III secretion genes in Aeromonas salmonicida subsp salmonicida are located on a large thermolabile virulence plasmid.杀鲑气单胞菌杀鲑亚种中的III型分泌基因位于一个大型热不稳定毒力质粒上。
J Clin Microbiol. 2003 Aug;41(8):3854-6. doi: 10.1128/JCM.41.8.3854-3856.2003.
6
Evidence for a type III secretion system in Aeromonas salmonicida subsp. salmonicida.杀鲑气单胞菌杀鲑亚种中III型分泌系统的证据。
J Bacteriol. 2002 Nov;184(21):5966-70. doi: 10.1128/JB.184.21.5966-5970.2002.
7
Novel Aeromonas hydrophila PPD134/91 genes involved in O-antigen and capsule biosynthesis.参与O抗原和荚膜生物合成的新型嗜水气单胞菌PPD134/91基因。
Infect Immun. 2002 May;70(5):2326-35. doi: 10.1128/IAI.70.5.2326-2335.2002.
8
Extended method for discrimination of Aeromonas spp. by 16S rDNA RFLP analysis.通过16S rDNA RFLP分析鉴别气单胞菌属的扩展方法。
Int J Syst Evol Microbiol. 2000 Nov;50 Pt 6:2069-2073. doi: 10.1099/00207713-50-6-2069.
9
Assembly and function of type III secretory systems.III型分泌系统的组装与功能。
Annu Rev Microbiol. 2000;54:735-74. doi: 10.1146/annurev.micro.54.1.735.
10
Clinically relevant Aeromonas species.具有临床相关性的气单胞菌属菌种。
Clin Infect Dis. 2000 Jun;30(6):988-9. doi: 10.1086/313839.
Zotero 插件