Tsuzuki Tsuyoshi, Ikeda Ikuo
Department of Food Management, School of Food, Agricultural and Environmental Sciences, Miyagi University, Sendai, Japan.
Biosci Biotechnol Biochem. 2007 Aug;71(8):2034-40. doi: 10.1271/bbb.70238. Epub 2007 Aug 7.
We have previously shown that the 9c,11t-conjugated linoleic acid (CLA) concentration was always significantly higher than the 10t,12c-CLA concentration following the administration of these compounds to mice and rats, and considered that structural differences between the conjugated double bonds in these isomers affected absorption in the small intestine. This study investigates the absorption of CLA in the rat intestine by a lipid absorption assay of lymph from the thoracic duct. In Study 1, we used safflower oil and a triacylglycerol form of CLA (CLA-TG), while in Study 2, we used 9c,11t-CLA and 10t,12c-CLA. The cumulative recovery of CLA was lower than that of linoleic acid until two hours after sample administration. There was no difference in the extent of lymphatic recovery of 9c,11t-CLA and 10t,12c-CLA after the administration of CLA-TG, 9c,11t-CLA, and 10t,12c-CLA to the rats, suggesting that geometrical and positional isomerism of the conjugated double bonds did not influence the absorption.
我们之前已经表明，在给小鼠和大鼠施用这些化合物后，9c,11t-共轭亚油酸（CLA）的浓度总是显著高于10t,12c-CLA的浓度，并认为这些异构体中共轭双键的结构差异影响了小肠中的吸收。本研究通过胸导管淋巴的脂质吸收测定法研究了CLA在大鼠肠道中的吸收情况。在研究1中，我们使用了红花油和CLA的三酰甘油形式（CLA-TG），而在研究2中，我们使用了9c,11t-CLA和10t,12c-CLA。在给药后两小时内，CLA的累积回收率低于亚油酸。在给大鼠施用CLA-TG、9c,11t-CLA和10t,12c-CLA后，9c,11t-CLA和10t,12c-CLA的淋巴回收率没有差异，这表明共轭双键的几何和位置异构不影响吸收。
