Mosqueda J, Figueroa J V, Alvarez A, Bautista R, Falcon A, Ramos A, Canto G, Vega C A
Bovine Babesiosis Laboratory, National Center for Disciplinary Research in Veterinary Parasitology (CENID-PAVET)-INIFAP, Jiutepec, Morelos, Mexico.
Parassitologia. 2007 May;49 Suppl 1:19-22.
The severe negative impact that bovine babesiosis has in the Mexican cattle industry has not been ameliorated basically due to the lack of safe and effective commercially available vaccines and sensitive and reliable diagnostic tests. In recent years, the Bovine Babesiosis Laboratory at the National Center for Disciplinary Research in Veterinary Parasitology-INIFAP in Morelos State, Mexico has been directing efforts towards three main research areas: (1) The development of in vitro culture-derived, improved and safer live vaccines. This has been done in two ways: using gamma-irradiated bovine serum and erythrocytes for the in vitro culture of vaccine strains, which reduces the risk of contaminating pathogens, and improving the immune response, by the addition of L. casei, a strong stimulant of the innate immune system. (2) The study of antigens considered as vaccine candidates with the goal of developing a recombinant vaccine that suits the country's needs. Knowing their degree of conservation or variation in Mexican isolates, their phylogenetic relationship and their protective, immuno-stimulatory properties, are first steps towards that goal. (3) The development of new tools for diagnosis, detection and discrimination of bovine babesiosis is the third area. Developing variants of ELISA, which are more reliable than the currently used IFAT, are a priority, and finally, taking advantage of the genomes of Babesia bigemina, and B. bovis, we are identifying genes than allow us to discriminate isolates using molecular tools.
牛巴贝斯虫病对墨西哥养牛业造成的严重负面影响基本未得到改善，这主要是由于缺乏安全有效的商用疫苗以及灵敏可靠的诊断检测方法。近年来，位于墨西哥莫雷洛斯州的国家兽医寄生虫学学科研究中心-INIFAP的牛巴贝斯虫病实验室一直致力于三个主要研究领域：（1）开发体外培养衍生的、改良且更安全的活疫苗。这通过两种方式实现：使用经γ射线辐照的牛血清和红细胞进行疫苗株的体外培养，降低病原体污染风险；通过添加干酪乳杆菌（一种先天免疫系统的强刺激剂）来改善免疫反应。（2）研究被视为候选疫苗的抗原，目标是开发出符合该国需求的重组疫苗。了解它们在墨西哥分离株中的保守程度或变异情况、它们的系统发育关系以及它们的保护性、免疫刺激特性，是朝着该目标迈出的第一步。（3）开发用于诊断、检测和鉴别牛巴贝斯虫病的新工具是第三个领域。开发比目前使用的间接荧光抗体试验（IFAT）更可靠的酶联免疫吸附测定（ELISA）变体是当务之急，最后，利用双芽巴贝斯虫和牛巴贝斯虫的基因组，我们正在鉴定能够让我们使用分子工具鉴别分离株的基因。
