Mercado-Uriostegui Miguel Angel, Castro-Sánchez Luis Alberto, Batiha Gaber El-Saber, Valdez-Espinoza Uriel Mauricio, Falcón-Neri Alfonso, Ramos-Aragon Juan Alberto, Hernández-Ortiz Ruben, Kawazu Shin-Ichiro, Igarashi Ikuo, Mosqueda Juan
Immunology and Vaccines Laboratory, College of Natural Sciences, Autonomous University of Queretaro, Santiago de Querétaro 76140, Querétaro, Mexico.
Programa de Doctorado en Ciencias biológicas, Facultad de Ciencias Naturales, Universidad Autónoma de Querétaro, Juriquilla 76230, Querétaro, Mexico.
Pathogens. 2022 May 18;11(5):591. doi: 10.3390/pathogens11050591.
In , the 45 kilodaltons glycoprotein (GP-45) is the most studied. GP-45 is exposed on the surface of the merozoite, it is believed to play a role in the invasion of erythrocytes, and it is characterized by a high genetic and antigenic polymorphism. The objective of this study was to determine if GP-45 contains conserved B-cell epitopes, and if they would induce neutralizing antibodies. The comparative analysis of nucleotide and amino acids sequences revealed a high percentage of similarity between field isolates. Antibodies against peptides containing conserved B-cell epitopes of GP-45 were generated. Antibodies present in the sera of mice immunized with GP-45 peptides specifically recognize by the IFAT. More than 95% of cattle naturally infected with contained antibodies against conserved GP-45 peptides tested by ELISA. Finally, sera from rabbits immunized with GP-45 peptides were evaluated in vitro neutralization tests and it was shown that they reduced the percentage of parasitemia compared to sera from rabbits immunized with adjuvant. GP-45 from geographically distant isolates of contains conserved B-cell epitopes that induce neutralizing antibodies suggesting that this gene and its product play a critical role in the survival of the parasite under field conditions.
在[某种寄生虫]中，45千道尔顿糖蛋白（GP - 45）是研究最多的。GP - 45暴露于裂殖子表面，据信它在红细胞入侵中起作用，并且其特点是具有高度的遗传和抗原多态性。本研究的目的是确定GP - 45是否含有保守的B细胞表位，以及它们是否会诱导中和抗体。核苷酸和氨基酸序列的比较分析显示，野外分离株之间具有很高的相似百分比。产生了针对含有GP - 45保守B细胞表位的肽的抗体。用GP - 45肽免疫的小鼠血清中的抗体通过间接荧光抗体试验（IFAT）特异性识别[相关抗原]。通过酶联免疫吸附测定（ELISA）检测，超过95%自然感染[该寄生虫]的牛含有针对保守的GP - 45肽的抗体。最后，在体外中和试验中评估了用GP - 45肽免疫的兔子的血清，结果表明与用佐剂免疫的兔子的血清相比，它们降低了寄生虫血症的百分比。来自地理上遥远的[该寄生虫]分离株的GP - 45含有保守的B细胞表位，这些表位可诱导中和抗体，这表明该基因及其产物在野外条件下寄生虫的存活中起关键作用。
