Lin Peng, Xia Lixin, Ng T B
Department of Biochemistry, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.
Peptides. 2007 Aug;28(8):1514-9. doi: 10.1016/j.peptides.2007.06.028. Epub 2007 Jul 10.
An antifungal peptide with a molecular mass of 9412 and an N-terminal sequence exhibiting notable homology to those of lipid transfer proteins was isolated from seeds of the vegetable Brassica campestris. The purification protocol entailed ion exchange chromatography on Q-Sepharose, affinity chromatography on Affi-gel blue gel, ion exchange chromatography by fast protein liquid chromatography (FPLC) on Mono S, and gel filtration by FPLC on a Superdex peptide column. The antifungal peptide was adsorbed on Affi-gel blue gel and Mono S. It inhibited mycelial growth in Fusarium oxysporum and Mycosphaerella arachidicola with an IC(50) value of 8.3 microM and 4.5 microM, respectively. It exhibited dose-dependent binding to lyso-alpha-lauroyl phosphatidylcholine. The present findings constitute the first report on a non-specific lipid transfer protein from the seeds of a Brassica species.
从蔬菜油菜籽中分离出一种分子量为9412的抗真菌肽，其N端序列与脂质转移蛋白的N端序列具有显著同源性。纯化方案包括在Q-Sepharose上进行离子交换色谱、在Affi-gel蓝胶上进行亲和色谱、在Mono S上通过快速蛋白质液相色谱（FPLC）进行离子交换色谱以及在Superdex肽柱上通过FPLC进行凝胶过滤。该抗真菌肽吸附在Affi-gel蓝胶和Mono S上。它分别以8.3 microM和4.5 microM的IC(50)值抑制尖孢镰刀菌和落花生球腔菌的菌丝生长。它表现出与溶血-α-月桂酰磷脂酰胆碱的剂量依赖性结合。本研究结果构成了关于芸苔属植物种子中非特异性脂质转移蛋白的首次报道。
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