Department of Biochemistry, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.
J Biosci Bioeng. 2009 Sep;108(3):259-65. doi: 10.1016/j.jbiosc.2009.03.013.
An 9.4-kDa antifungal peptide designated as campesin was isolated from seeds of the cabbage Brassica campestris. The isolation procedure involved affinity chromatography on Affi-gel blue gel, ion exchange chromatography on Q-Sepharose and Mono S, and gel filtration on Superdex 75 and Superdex Peptide. The peptide was adsorbed on the first three chromatographic media. It exerted an inhibitory action on mycelial growth including Fusarium oxysporum and Mycosphaerella arachidicola, with an IC(50) of 5.1 microM and 4.4 microM, respectively. The peptide was characterized by remarkable thermostability and pH stability. It inhibited proliferation of HepG2 and MCF cancer cells with an IC(50) of 6.4 microM and 1.8 microM, and the activity of HIV-1 reverse transcriptase with an IC(50) of 3.2 microM. It demonstrated lysolecithin binding activity.
一种 9.4kDa 的抗真菌肽，被命名为 campesin，从甘蓝型油菜 Brassica campestris 的种子中分离得到。该分离过程包括：Affi-gel blue 凝胶的亲和层析、Q-Sepharose 和 Mono S 的离子交换层析，以及 Superdex 75 和 Superdex Peptide 的凝胶过滤。该肽可被前三款层析介质吸附。它对包括尖孢镰刀菌和拟茎点霉在内的菌丝生长具有抑制作用，IC(50)分别为 5.1μM 和 4.4μM。该肽具有显著的热稳定性和 pH 稳定性。它对 HepG2 和 MCF 癌细胞的增殖具有抑制作用，IC(50)分别为 6.4μM 和 1.8μM，对 HIV-1 逆转录酶的活性具有抑制作用，IC(50)为 3.2μM。它具有溶血磷脂结合活性。
