de Morais Helio A, Guimarães Ana Marcia S, Vidotto Odilon, Baumann Aline, Biondo Alexander W, Messick Joanne B
Department of Medical Sciences, University of Wisconsin, Madison, WI 43210, USA.
J Feline Med Surg. 2007 Dec;9(6):518-20. doi: 10.1016/j.jfms.2007.05.005. Epub 2007 Aug 10.
The two most common haemotropic Mycoplasma of cats, Mycoplasma haemofelis and 'Candidatus Mycoplasma haemominutum' have been identified using molecular techniques in all continents, except Antarctica. We report the first molecular characterization in South America of a dual infection with M haemofelis and 'Candidatus Mycoplasma haemominutum' in three domestic cats. The 16S ribosomal RNA gene was amplified in three anaemic cats in which haemoplasma organisms were seen attached to the erythrocytes in the peripheral blood smear. Bands of the expected size for M haemofelis and 'Candidatus Mycoplasma haemominutum' were observed in all three cats. The 393 bp segment of one of the amplicons had a similarity value of 100% to M haemofelis, whereas the other amplicon, a 192 bp segment, was 100% similar to 'Candidatus Mycoplasma haemominutum'. After diagnosis, two cats received blood transfusion and they were all treated with doxycycline. All three cats recovered uneventfully.
猫的两种最常见的嗜血性支原体,即溶血支原体(Mycoplasma haemofelis)和“溶血微小支原体(暂定种)”('Candidatus Mycoplasma haemominutum'),已通过分子技术在除南极洲以外的各大洲被鉴定出来。我们报告了在南美洲首次对三只家猫进行的溶血支原体和“溶血微小支原体(暂定种)”双重感染的分子特征分析。在三只贫血猫中扩增出了16S核糖体RNA基因,在外周血涂片中可见血支原体附着于红细胞上。在所有三只猫中均观察到了溶血支原体和“溶血微小支原体(暂定种)”预期大小的条带。其中一个扩增子的393bp片段与溶血支原体的相似性值为100%,而另一个192bp片段的扩增子与“溶血微小支原体(暂定种)”的相似性为100%。诊断后,两只猫接受了输血治疗,并且它们都接受了强力霉素治疗。所有三只猫均顺利康复。