丹麦猫中猫血巴尔通体的患病率。
Prevalence of feline haemoplasma in cats in Denmark.
作者信息
Rosenqvist Maja Benedicte, Meilstrup Ann-Katrine Helene, Larsen Jesper, Olsen John Elmerdahl, Jensen Asger Lundorff, Thomsen Line Elnif
机构信息
Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Stigboejlen 4, 1870, Frederiksberg C, Denmark.
Department of Veterinary Clinical and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Dyrlaegevej 16, 1870, Frederiksberg C, Denmark.
出版信息
Acta Vet Scand. 2016 Nov 10;58(1):78. doi: 10.1186/s13028-016-0260-1.
BACKGROUND
Infections with the three feline haemotropic mycoplasmas Mycoplasma haemofelis, Candidatus Mycoplasma haemominutum and Candidatus Mycoplasma turicensis cause feline infectious anemia. The purpose of this study was to investigate the prevalence of carriage of feline haemoplasma in Danish cats in different age groups. The presence was detected by a conventional polymerase chain reaction (PCR) assay on blood samples as well as by real-time PCR (RT-PCR).
RESULTS
The study revealed a prevalence of 14.9% Candidatus Mycoplasma haemominutum positive cats and 1.5% Mycoplasma haemofelis positive cats. No cats were found positive for Candidatus Mycoplasma turicensis. The results showed a statistically significant higher prevalence in older (>8 years) cats compared to younger cats and a higher prevalence among domestic cats compared to purebred cats. As part of this study, we developed a cloning strategy to obtain Danish positive controls of haemoplasma 16S rRNA.
CONCLUSION
From convenience-sampled cats in Denmark, we found that 16.4% were carriers of feline haemotropic mycoplasmas. Haemoplasma was mostly found in older and domestic cats. The prevalence found in Denmark is similar to that found in several other European countries.
背景
感染三种猫血支原体，即溶血支原体（Mycoplasma haemofelis）、拟血支原体（Candidatus Mycoplasma haemominutum）和图氏拟血支原体（Candidatus Mycoplasma turicensis）会导致猫传染性贫血。本研究的目的是调查丹麦不同年龄组猫携带猫血支原体的流行情况。通过对血液样本进行常规聚合酶链反应（PCR）检测以及实时PCR（RT-PCR）来检测其存在情况。
结果
该研究发现，拟血支原体阳性猫的流行率为14.9%，溶血支原体阳性猫的流行率为1.5%。未发现图氏拟血支原体阳性猫。结果显示，与年轻猫相比，老年（>8岁）猫的流行率在统计学上显著更高，与纯种猫相比，家猫中的流行率更高。作为本研究的一部分，我们开发了一种克隆策略，以获得丹麦血支原体16S rRNA的阳性对照。
结论
从丹麦方便抽样的猫中，我们发现16.4%是猫血支原体的携带者。血支原体大多在老年猫和家猫中发现。在丹麦发现的流行率与其他几个欧洲国家相似。
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