Palmer S J, Burgoyne P S
MRC Mammalian Development Unit, London, UK.
Development. 1991 May;112(1):265-8. doi: 10.1242/dev.112.1.265.
The testes of fetal, prepuberal and adult XX----XY chimaeras were examined using in situ hybridisation to identify the beta-globin transgenic marker contained in one component of each chimaera. This enabled the proportion of XX and XY cells contributing to the major cell lineages of the testis to be estimated from sectioned and air-dried material. A few XX Sertoli cells were found in all three age groups, but the XX contribution was always much lower than in other somatic cell types. Significantly, in fetal XX----XY testes, Sertoli cells were the only cell type to show a bias in favour of the XY component. This strengthens the view that Tdy acts solely in the lineage that gives rise to Sertoli cells. However, the finding of some fetal XX Sertoli cells means that one of the steps in the Tdy-initiated process of Sertoli cell determination is capable of locally recruiting XX cells.
利用原位杂交技术对胎儿、青春期前及成年XX----XY嵌合体的睾丸进行检查，以识别每个嵌合体其中一个成分中所含的β-珠蛋白转基因标记。这使得能够从切片和风干材料中估计出对睾丸主要细胞谱系有贡献的XX和XY细胞的比例。在所有三个年龄组中均发现了少量XX支持细胞，但XX细胞的贡献始终远低于其他体细胞类型。值得注意的是，在胎儿XX----XY睾丸中，支持细胞是唯一偏向XY成分的细胞类型。这强化了Tdy仅在产生支持细胞的谱系中起作用的观点。然而，发现一些胎儿XX支持细胞意味着，Tdy启动的支持细胞决定过程中的一个步骤能够在局部招募XX细胞。
