城市社区新鲜农产品中高毒力克隆复合体（CC）
Hypervirulent clonal complex (CC) of in fresh produce from urban communities.
作者信息
Amarasekara Nirosha Ruwani, Swamy Amrita Subramanya, Paudel Sumit Kumar, Jiang Wentao, Li KaWang, Shen Cangliang, Zhang Yifan
机构信息
Department of Nutrition and Food Science, Wayne State University, Detroit, MI, United States.
Davis College, Division of Animal and Nutritional Sciences, West Virginia University, Morgantown, WV, United States.
出版信息
Front Microbiol. 2024 Jan 29;15:1307610. doi: 10.3389/fmicb.2024.1307610. eCollection 2024.
INTRODUCTION
This study aimed to determine the prevalence and virulome of in fresh produce distributed in urban communities.
METHODS
A total of 432 fresh produce samples were collected from farmer's markets in Michigan and West Virginia, USA, resulting in 109 pooled samples. spp. were isolated and was subjected to genoserogrouping by PCR and genotyping by pulsed-field gel electrophoresis (PFGE). Multi-locus sequence typing (MLST) and core-genome multi-locus sequence typing (cgMLST) were conducted for clonal identification.
RESULTS
Forty-eight of 109 samples (44.0%) were contaminated with spp. serotype 1/2a and 4b were recovered from radishes, potatoes, and romaine lettuce. Four clonal complexes (CC) were identified and included hypervirulent CC1 (ST1) and CC4 (ST219) of lineage I as well as CC7 (ST7) and CC11 (ST451) of lineage II. Clones CC4 and CC7 were present in the same romaine lettuce sample. CC1 carried pathogenicity island LIPI-1 and LIPI-3 whereas CC4 contained LIPI-1, LIPI-3, and LIPI-4. CC7 and CC11 had LIPI-1 only.
DISCUSSION
Due to previous implication in outbreaks, hypervirulent clones in fresh produce pose a public health concern in urban communities.
引言
本研究旨在确定城市社区中新鲜农产品上 的流行情况和毒力组。
方法
从美国密歇根州和西弗吉尼亚州的农贸市场收集了总共432份新鲜农产品样本，得到109个混合样本。分离出 菌株，并通过聚合酶链反应（PCR）进行血清群分型，通过脉冲场凝胶电泳（PFGE）进行基因分型。进行多位点序列分型（MLST）和核心基因组多位点序列分型（cgMLST）以进行克隆鉴定。
结果
109个样本中有48个（44.0%）被 菌株污染。从萝卜、土豆和长叶生菜中分离出1/2a和4b血清型。鉴定出四个克隆复合体（CC），包括I系的高毒力CC1（ST1）和CC4（ST219）以及II系的CC7（ST7）和CC11（ST451）。克隆CC4和CC7存在于同一个长叶生菜样本中。CC1携带致病性岛LIPI-1和LIPI-3，而CC4包含LIPI-1、LIPI-3和LIPI-4。CC7和CC11仅含有LIPI-1。
讨论
由于之前与疫情爆发有关，新鲜农产品中的 高毒力克隆对城市社区的公共卫生构成了威胁。
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