Remedios Ryan, Huilgol Dhananjay, Saha Bhaskar, Hari Padmanabhan, Bhatnagar Lahar, Kowalczyk Thomas, Hevner Robert F, Suda Yoko, Aizawa Shinichi, Ohshima Toshio, Stoykova Anastassia, Tole Shubha
Department of Biological Sciences, B-304, Tata Institute of Fundamental Research, Homi Bhabha Road, Colaba, Mumbai 400005, India.
Nat Neurosci. 2007 Sep;10(9):1141-50. doi: 10.1038/nn1955. Epub 2007 Aug 12.
The amygdaloid complex consists of diverse nuclei that belong to distinct functional systems, yet many issues about its development are poorly understood. Here, we identify a stream of migrating cells that form specific amygdaloid nuclei in mice. In utero electroporation showed that this caudal amygdaloid stream (CAS) originated in a unique domain at the caudal telencephalic pole that is contiguous with the dorsal pallium, which was previously thought to generate only neocortical cells. The CAS and the neocortex share mechanisms for specification (transcription factors Tbr1, Lhx2 and Emx1/2) and migration (reelin and Cdk5). Reelin, a critical cue for migration in the neocortex, and Cdk5, which is specifically required for migration along radial glia in the neocortex, were both selectively required for the normal migration of the CAS, but not for that of other amygdaloid nuclei. This is first evidence of a dorsal pallial contribution to the amygdala, demonstrating a developmental and mechanistic link between the amygdala and the neocortex.
杏仁核复合体由属于不同功能系统的多种核团组成，然而关于其发育的许多问题仍知之甚少。在此，我们在小鼠中鉴定出一股迁移细胞流，它们形成特定的杏仁核核团。子宫内电穿孔显示，这条尾侧杏仁核流（CAS）起源于尾侧端脑极的一个独特区域，该区域与背侧皮层相邻，而背侧皮层此前被认为仅产生新皮层细胞。CAS和新皮层在特化（转录因子Tbr1、Lhx2和Emx1/2）和迁移（Reelin和Cdk5）方面具有共同机制。Reelin是新皮层迁移的关键信号，Cdk5是新皮层中沿放射状胶质细胞迁移所特别需要的，二者都是CAS正常迁移所选择性需要的，但对其他杏仁核核团的迁移则不是必需的。这是背侧皮层对杏仁核有贡献的首个证据，证明了杏仁核与新皮层之间的发育和机制联系。
