烟草与炎症对口腔黏膜上皮中hMSH2和hMLH1免疫表达的影响。
Tobacco and inflammation effects in immunoexpression of hMSH2 and hMLH1 in epithelium of oral mucosa.
作者信息
Fernandes Anacélia Mendes, De Souza Vânia Ribeiro da Cunha, Springer Cornelis Robert Araújo, Cardoso Sérgio Vitorino, Loyola Adriano Mota, Mesquita Ricardo Alves, Aguiar Maria Cássia Ferreira
机构信息
Department of Oral Pathology and Surgery, School of Dentistry, Universidade Federal de Minas Gerais, Brazil.
出版信息
Anticancer Res. 2007 Jul-Aug;27(4B):2433-7.
BACKGROUND
Both hMSH2 and hMLH1 are integrated in the DNA mismatch repair (MMR) system. Reduced expression of these proteins has been reported in head and neck carcinoma; however, few studies have examined the normal levels of these proteins in oral tissues or investigated factors which could interfere with this expression. The aim of this study was to detect the expression pattern of hMSH2 and hMLH1 in normal oral mucosa and to correlate this with demographic data, smoking and local inflammation.
MATERIALS AND METHODS
The expression of hMSH2 and hMLH1 was evaluated in 38 samples of normal mucosa epithelium using immunohistochemistry.
RESULTS
No relationship was observed between hMSH2 and hMLH1 and demographic data. The hMLH1 but not hMSH2 expression was altered in smokers and in the presence of inflammation.
CONCLUSION
Both hMSH2 and hMLH1 are highly expressed in the normal epithelium of oral mucosa. In addition, hMLH1 expression is directly influenced by tobacco use and inflammation.
背景
hMSH2和hMLH1均参与DNA错配修复（MMR）系统。已有报道称这些蛋白在头颈癌中表达降低；然而，很少有研究检测这些蛋白在口腔组织中的正常水平，或研究可能干扰其表达的因素。本研究的目的是检测hMSH2和hMLH1在正常口腔黏膜中的表达模式，并将其与人口统计学数据、吸烟和局部炎症相关联。
材料与方法
采用免疫组织化学方法评估38例正常黏膜上皮样本中hMSH2和hMLH1的表达。
结果
未观察到hMSH2和hMLH1与人口统计学数据之间存在关联。吸烟者以及存在炎症时，hMLH1的表达发生改变，而hMSH2未改变。
结论
hMSH2和hMLH1在正常口腔黏膜上皮中均高表达。此外，hMLH1的表达直接受烟草使用和炎症的影响。
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