Wang Yong-Zhong, Wu Guo-Xiang, Luo Li-Bo, Chen Min, Ruan Li-Hua
Research Center for Liver diseases, The Third Hospital of Changzhou, Changzhou 213001, Jiangsu Province, China.
World J Gastroenterol. 2007 Aug 21;13(31):4260-3. doi: 10.3748/wjg.v13.i31.4260.
To compare the oligonucleotide chip, real-time PCR and sequencing for genotyping of hepatitis B virus in Chinese patients with chronic hepatitis B.
Mixture of samples with different genotypes and clinical serum samples from 126 chronic hepatitis B patients was tested for hepatitis B virus genotypes by oligonucleotide chip, real-time PCR and sequencing of PCR products, respectively. Clinical performances, time required and costs of the three assays were evaluated.
Oligonucleotide chips and real-time PCR detected 1% and 0.1% genotypes, respectively, in mixed samples. Of the 126 clinical samples from patients with chronic hepatitis B, genotype B was detected in 41 (33%), 41 (33%) and 45 (36%) samples, and genotype C in 76 (60%), 76 (60%) and 81 (64%) samples, by oligonucleotide chip, real-time PCR and sequencing, respectively. Oligonucleotide chip and real-time PCR detected mixed genotypes B and C in 9 samples. Real-time PCR was the rapidest and cheapest among the three assays.
Oligonucleotide chip and real-time PCR are able to detect mixed genotypes, while sequencing only detects the dominant genotype in clinical samples.
比较寡核苷酸芯片、实时荧光定量聚合酶链反应（PCR）及测序法对中国慢性乙型肝炎患者乙肝病毒基因分型的效果。
分别采用寡核苷酸芯片、实时荧光定量PCR及PCR产物测序法，对含不同基因型的混合样本以及126例慢性乙型肝炎患者的临床血清样本进行乙肝病毒基因分型检测，并对这三种检测方法的临床性能、所需时间及成本进行评估。
在混合样本中，寡核苷酸芯片和实时荧光定量PCR分别检测出1%和0.1%的基因型。在126例慢性乙型肝炎患者的临床样本中，寡核苷酸芯片、实时荧光定量PCR及测序法分别检测出41例（33%）、41例（33%）和45例（36%）B基因型样本，以及76例（60%）、76例（60%）和81例（64%）C基因型样本。寡核苷酸芯片和实时荧光定量PCR在9个样本中检测出B和C基因型混合情况。实时荧光定量PCR是三种检测方法中速度最快且成本最低的。
寡核苷酸芯片和实时荧光定量PCR能够检测出混合基因型，而测序法仅能检测出临床样本中的优势基因型。
