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Enzymatic synthesis of fluorinated mechanistic probes for sialidases and sialyltransferases.

作者信息

Chokhawala Harshal A, Cao Hongzhi, Yu Hai, Chen Xi

机构信息

Department of Chemistry, University of California, One Shields Avenue, Davis, California 95616, USA.

出版信息

J Am Chem Soc. 2007 Sep 5;129(35):10630-1. doi: 10.1021/ja072687u. Epub 2007 Aug 14.

DOI:10.1021/ja072687u
PMID:17696347
Abstract
摘要

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Enzymatic synthesis of fluorinated mechanistic probes for sialidases and sialyltransferases.
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Demonstration of sialyl transfer from exogenous CMP-N-acetyl-[14C]neuraminic acid to cell membrane-bound acceptor molecules at the surface of intact Ehrlich ascites tumor cells.在外源CMP-N-乙酰-[¹⁴C]神经氨酸向完整艾氏腹水肿瘤细胞表面的细胞膜结合受体分子进行唾液酸转移的证明。
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Metabolism of N-acetylneuraminic acid in mammals: isolation and characterization of CMP-N-acetylneuraminic acid.哺乳动物中N-乙酰神经氨酸的代谢:CMP-N-乙酰神经氨酸的分离与特性鉴定
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Sialic acid activation.唾液酸激活
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Enzymatic synthesis of sialylation substrates powered by a novel polyphosphate kinase (PPK3).由新型多聚磷酸激酶(PPK3)驱动的唾液酸化底物的酶促合成。
Org Biomol Chem. 2009 May 7;7(9):1778-80. doi: 10.1039/b822549b. Epub 2009 Mar 23.

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