Zhang Lihua, Liu Baifeng, Dong Shaojun
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Graduate School of the Chinese Academy of Sciences, Chinese Academy of Sciences, Changchun, 130022, China.
J Phys Chem B. 2007 Sep 6;111(35):10448-52. doi: 10.1021/jp0734427. Epub 2007 Aug 15.
Bifunctional nanoarchitecture has been developed by combining the magnetic iron oxide and the luminescent Ru(bpy)32+ encapsulated in silica. First, the iron oxide nanoparticles were synthesized and coated with silica, which was used to isolate the magnetic nanoparticles from the outer-shell encapsulated Ru(bpy)32+ to prevent luminescence quenching. Then onto this core an outer shell of silica containing encapsulated Ru(bpy)32+ was grown through the Stöber method. Highly luminescent Ru(bpy)32+ serves as a luminescent marker, while magnetic Fe3O4 nanoparticles allow external manipulation by a magnetic field. Since Ru(bpy)32+ is a typical electrochemiluminescence (ECL) reagent and it could still maintain such property when encapsulated in the bifunctional nanoparticle, we explored the feasibility of applying the as-prepared nanostructure to fabricating an ECL sensor; such method is simple and effective. We applied the prepared ECL sensor not only to the typical Ru(bpy)32+ co-reactant tripropylamine (TPA), but also to the practically important polyamines. Consequently, the ECL sensor shows a wide linear range, high sensitivity, and good stability.
通过将磁性氧化铁与封装在二氧化硅中的发光钌（联吡啶）32+相结合，开发出了双功能纳米结构。首先，合成了氧化铁纳米颗粒并用二氧化硅包覆，这用于将磁性纳米颗粒与外壳封装的钌（联吡啶）32+隔离开来，以防止发光猝灭。然后，通过施托伯方法在这个核心上生长一层含有封装钌（联吡啶）32+的二氧化硅外壳。高发光性的钌（联吡啶）32+用作发光标记物，而磁性Fe3O4纳米颗粒允许通过磁场进行外部操控。由于钌（联吡啶）32+是一种典型的电化学发光（ECL）试剂，并且当封装在双功能纳米颗粒中时仍能保持这种性质，我们探索了将所制备的纳米结构应用于制造ECL传感器的可行性；这种方法简单有效。我们将所制备的ECL传感器不仅应用于典型的钌（联吡啶）32+共反应剂三丙胺（TPA），还应用于实际重要的多胺。因此，该ECL传感器显示出宽线性范围、高灵敏度和良好的稳定性。
