Lai Long-Shan T, Hung Chih-Sheng, Lo Chi-Chu
Department of Applied Chemistry, Chaoyang University of Technology, Wufong, Taichung County 413, Taiwan, ROC.
J Biosci Bioeng. 2007 Jul;104(1):9-13. doi: 10.1263/jbb.104.9.
Fermentation products of Aspergillus terreus ATCC 20542 (a parent strain for lovastatin production) were collected, and the coexistence of itaconic acid (IA) with lovastatin was confirmed in this study. Using a lactose-based medium (LBM), lovastatin production was 873 mg/l on day 10, but IA production was only 22-28 mg/l during the cultures. When lactose in LBM was simply replaced with glucose, IA production was markedly enhanced by 20-fold (491 mg/l on day 5), which showed a growth-associated pattern. The findings indicated that the carbon source used (glucose or lactose) controlled the biosynthetic pathway. The net yield of lovastatin production when using lactose was calculated to be 25.1 mg/g (5.1-fold) in comparison with when using glucose in the cultures. Furthermore, lovastatin production was further increased by 9.2% when IA (0.5 g/l) was added to LBM. When IA was added at 5 g/l, the fermentation broth turned dark-brown, and lovastatin production was reduced by 18.0%. Hence, these two metabolites (IA and lovastatin) produced by the fungus might be related.
收集了土曲霉ATCC 20542（洛伐他汀生产的亲本菌株）的发酵产物，本研究证实了衣康酸（IA）与洛伐他汀共存。使用基于乳糖的培养基（LBM），第10天洛伐他汀产量为873 mg/l，但培养期间IA产量仅为22 - 28 mg/l。当LBM中的乳糖简单地被葡萄糖替代时，IA产量显著提高了20倍（第5天为491 mg/l），呈现出与生长相关的模式。这些发现表明所使用的碳源（葡萄糖或乳糖）控制了生物合成途径。与在培养中使用葡萄糖相比，使用乳糖时洛伐他汀生产的净产量计算为25.1 mg/g（5.1倍）。此外，当向LBM中添加IA（0.5 g/l）时，洛伐他汀产量进一步提高了9.2%。当以5 g/l添加IA时，发酵液变成深棕色，洛伐他汀产量降低了18.0%。因此，该真菌产生的这两种代谢产物（IA和洛伐他汀）可能存在关联。
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