Ogushi Yuko, Sakai Shinji, Kawakami Koei
Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 744 Motooka, Fukuoka, Japan.
J Biosci Bioeng. 2007 Jul;104(1):30-3. doi: 10.1263/jbb.104.30.
We synthesized a carboxymethylcellulose with phenol moieties by covalently incorporating tyramine into carboxymethylcellulose using aqueous-phase carbodiimide activation chemistry. The resulting hydrogel was obtained from an aqueous solution of the conjugate via the horseradish peroxidase-catalyzed oxidation reaction of phenols by consuming H(2)O(2), where the gelation speed depended on the concentrations of enzyme and H(2)O(2). The viability of the mammalian cells enclosed within the hydrogel prepared from 1.5% (w/v) conjugate solution containing 5 units/ml horseradish peroxidase and 1 mM H(2)O(2), was 80% after 24 h. These results demonstrate that this carboxymethylcellulose with phenol moieties has potential for biomedical applications including tissue-engineering.
我们通过使用水相碳二亚胺活化化学方法将酪胺共价结合到羧甲基纤维素中，合成了一种带有酚基的羧甲基纤维素。通过辣根过氧化物酶催化酚类与消耗的H(2)O(2)发生氧化反应，从共轭物的水溶液中得到了所得水凝胶，其中凝胶化速度取决于酶和H(2)O(2)的浓度。在含有5单位/毫升辣根过氧化物酶和1毫摩尔H(2)O(2)的1.5%（重量/体积）共轭物溶液制备的水凝胶中包裹的哺乳动物细胞，24小时后的活力为80%。这些结果表明，这种带有酚基的羧甲基纤维素在包括组织工程在内的生物医学应用方面具有潜力。
