Yano Masato, Nagai Kouhei, Morimoto Koichi, Miyamoto Hiroshi
Wakayama Prefecture Collaboration of Regional Entities for the Advancement of Technological Excellence, Japan Science and Technology Agency, Japan.
Wakayama Prefecture Collaboration of Regional Entities for the Advancement of Technological Excellence, Japan Science and Technology Agency, Japan; Department of Biotechnological Science, School of Biology-Oriented Science and Technology, Kinki University, Nishimitani, Kinokawa, Wakayama 649-6493, Japan.
Biochem Biophys Res Commun. 2007 Oct 12;362(1):158-163. doi: 10.1016/j.bbrc.2007.07.172. Epub 2007 Aug 8.
A novel 19kDa protein, which was named N19, was isolated from the nacreous layer of the pearl oyster Pinctada fucata. N19 is one of predominant proteins found in the water-insoluble fraction of the nacreous layer. MALDI-TOF/TOF analysis indicated that the three trypsin-digested peptides (791.45, 824.42, and 1118.65m/z) corresponded to the amino acid sequences predicted from a cDNA isolated from a mantle cDNA library of P. fucata. Northern blot analysis revealed that the N19 mRNA was a little more abundant in the pallial region than the edge region, in the mantle. In CaCO(3) precipitation assay, the recombinant N19 protein inhibited the crystallization of CaCO(3). These results indicate that N19 is localized in the nacre and plays a negative regulatory role in calcification in the pearl oyster.
从合浦珠母贝珍珠层中分离出一种新的19kDa蛋白，命名为N19。N19是在珍珠层水不溶性组分中发现的主要蛋白质之一。基质辅助激光解吸电离飞行时间串联质谱（MALDI-TOF/TOF）分析表明，三种经胰蛋白酶消化的肽段（质荷比分别为791.45、824.42和1118.65）与从合浦珠母贝外套膜cDNA文库中分离出的一个cDNA所预测的氨基酸序列相对应。Northern印迹分析显示，N19 mRNA在外套膜的鳃区比边缘区略丰富。在碳酸钙沉淀试验中，重组N19蛋白抑制了碳酸钙的结晶。这些结果表明，N19定位于珍珠层，在合浦珠母贝的钙化过程中起负调控作用。
