Senyo Samuel E, Koshman Yevgeniya E, Russell Brenda
Department of Bioengineering, University of Illinois at Chicago, 835 S. Wolcott Avenue, Chicago, IL 60612, USA.
FEBS Lett. 2007 Sep 4;581(22):4241-7. doi: 10.1016/j.febslet.2007.07.070. Epub 2007 Aug 8.
The effect of interval, direction and rate of strain on mechanotransduction in neonatal rat cardiomyocytes is determined for focal adhesion kinase (Y397pFAK), extracellular signal-regulated kinase ERK1/2 (Thr(183)/Tyr(185)) and paxillin (pY31) and phosphorylation time courses to 10% strain assessed. Cells are non-responsive at 5 min but recover at 15 min (P<0.03) with FAK nuclear translocation by 30 min. Cyclic biaxial strain increased phosphorylation from slower to faster rates (P<0.05). Uniaxial strain to groove-aligned myocytes increased FAK and ERK1/2 phosphorylation transversely more than longitudinally (P<0.05). Mechanotransduction may have a refractory period of 5 min and differentiate directions and rates of strain.
测定了应变的间隔、方向和速率对新生大鼠心肌细胞机械转导的影响，评估了粘着斑激酶（Y397pFAK）、细胞外信号调节激酶ERK1/2（Thr(183)/Tyr(185)）和桩蛋白（pY31）以及磷酸化时间进程至10%应变。细胞在5分钟时无反应，但在15分钟时恢复（P<0.03），30分钟时FAK发生核转位。循环双轴应变使磷酸化速率从较慢增加到较快（P<0.05）。对沟对齐的心肌细胞施加单轴应变时，横向比纵向更能增加FAK和ERK1/2的磷酸化（P<0.05）。机械转导可能有5分钟的不应期，并能区分应变的方向和速率。
