Maeda S, Takayanagi K, Nishimura Y, Maruyama T, Sato K, Mizuno T
Laboratory of Microbiology, School of Agriculture, Nagoya University, Aichi.
J Biochem. 1991 Sep;110(3):324-7. doi: 10.1093/oxfordjournals.jbchem.a123579.
Expression of the Escherichia coli outer membrane proteins, OmpC and OmpF, is regulated in response to the medium osmolarity. The OmpR and EnvZ proteins are transcriptional factors involved in this osmotic regulation of the ompC and ompF genes. In particular, expression of the ompC gene is activated by the positive regulator, OmpR, in response to high osmolarity of the medium. In this study, we succeeded in defining a functional OmpR-binding sequence by analyzing a set of synthetic oligonucleotides, and propose a consensus motif for OmpR-binding. It was also demonstrated that the asymmetric OmpR-binding sequence, thus identified, can activate the canonical ompC promoter in an orientation independent-manner, providing that this sequence is placed closely and stereo-specifically with respect to the -35 region.
大肠杆菌外膜蛋白OmpC和OmpF的表达受培养基渗透压的调节。OmpR和EnvZ蛋白是参与ompC和ompF基因这种渗透调节的转录因子。特别地，ompC基因的表达在培养基高渗透压的响应下被正调控因子OmpR激活。在本研究中，我们通过分析一组合成寡核苷酸成功确定了一个功能性OmpR结合序列，并提出了一个OmpR结合的共有基序。还证明了如此鉴定出的不对称OmpR结合序列能够以方向独立的方式激活典型的ompC启动子，前提是该序列相对于-35区域紧密且立体特异性地定位。
