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大肠杆菌中ompC启动子上游激活序列存在多个OmpR结合位点的证据：单个OmpR结合位点即可激活该启动子。

Evidence for multiple OmpR-binding sites in the upstream activation sequence of the ompC promoter in Escherichia coli: a single OmpR-binding site is capable of activating the promoter.

作者信息

Maeda S, Mizuno T

机构信息

Laboratory of Microbiology, School of Agriculture, Nagoya University, Japan.

出版信息

J Bacteriol. 1990 Jan;172(1):501-3. doi: 10.1128/jb.172.1.501-503.1990.

DOI:10.1128/jb.172.1.501-503.1990
PMID:2403550
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC208463/
Abstract

We present experimental evidence for the existence of multiple activator-binding sites in the upstream sequence of the ompC promoter, the expression of which is activated by the positive regulator OmpR in response to the osmolarity of the medium. We also found that a single OmpR-binding site can activate the ompC promoter, providing that the binding site is close and placed stereospecifically with respect to the canonical-35 and -10 regions.

摘要

我们提供了实验证据，证明ompC启动子上游序列中存在多个激活剂结合位点，其表达由正调控因子OmpR响应培养基渗透压而激活。我们还发现，只要结合位点相对于典型的 -35和 -10区域紧密且立体特异性地放置，单个OmpR结合位点就能激活ompC启动子。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ad8/208463/38b9942c3a05/jbacter01043-0525-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ad8/208463/b958f8dab5ae/jbacter01043-0524-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ad8/208463/38b9942c3a05/jbacter01043-0525-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ad8/208463/b958f8dab5ae/jbacter01043-0524-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ad8/208463/38b9942c3a05/jbacter01043-0525-a.jpg

相似文献

1
Evidence for multiple OmpR-binding sites in the upstream activation sequence of the ompC promoter in Escherichia coli: a single OmpR-binding site is capable of activating the promoter.大肠杆菌中ompC启动子上游激活序列存在多个OmpR结合位点的证据：单个OmpR结合位点即可激活该启动子。
J Bacteriol. 1990 Jan;172(1):501-3. doi: 10.1128/jb.172.1.501-503.1990.
2
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Interaction of a transcriptional activator, OmpR, with reciprocally osmoregulated genes, ompF and ompC, of Escherichia coli.转录激活因子OmpR与大肠杆菌渗透压双向调节基因ompF和ompC的相互作用。
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Characterization by deletion and localized mutagenesis in vitro of the promoter region of the Escherichia coli ompC gene and importance of the upstream DNA domain in positive regulation by the OmpR protein.通过体外缺失和定位诱变对大肠杆菌ompC基因启动子区域进行表征以及上游DNA结构域在OmpR蛋白正调控中的重要性。
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本文引用的文献

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Regulation of oxidative stress response by CosR, an essential response regulator in Campylobacter jejuni.空肠弯曲菌中必需的应答调节子 CosR 对氧化应激反应的调控
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J Bacteriol. 2006 Jun;188(11):3731-9. doi: 10.1128/JB.01780-05.
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The Escherichia coli CpxA-CpxR envelope stress response system regulates expression of the porins ompF and ompC.大肠杆菌CpxA-CpxR包膜应激反应系统调控孔蛋白ompF和ompC的表达。
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J Biol Chem. 1981 Nov 25;256(22):11905-10.
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Positive control of transcription initiation in bacteria.细菌中转录起始的正调控
Annu Rev Genet. 1984;18:173-206. doi: 10.1146/annurev.ge.18.120184.001133.
5
Purification and characterization of the OmpR protein, a positive regulator involved in osmoregulatory expression of the ompF and ompC genes in Escherichia coli.大肠杆菌中参与ompF和ompC基因渗透调节表达的正向调节因子OmpR蛋白的纯化与特性分析
J Biol Chem. 1986 Nov 15;261(32):15252-6.
6
Characterization by deletion and localized mutagenesis in vitro of the promoter region of the Escherichia coli ompC gene and importance of the upstream DNA domain in positive regulation by the OmpR protein.通过体外缺失和定位诱变对大肠杆菌ompC基因启动子区域进行表征以及上游DNA结构域在OmpR蛋白正调控中的重要性。
J Bacteriol. 1986 Oct;168(1):86-95. doi: 10.1128/jb.168.1.86-95.1986.
7
Isolation and characterization of deletion mutants of ompR and envZ, regulatory genes for expression of the outer membrane proteins OmpC and OmpF in Escherichia coli.大肠杆菌中ompR和envZ缺失突变体的分离与鉴定，ompR和envZ是外膜蛋白OmpC和OmpF表达的调控基因。
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8
Activation of the ompC gene by the OmpR protein in Escherichia coli. The cis-acting upstream sequence can function in both orientations with respect to the canonical promoter.大肠杆菌中OmpR蛋白对ompC基因的激活作用。顺式作用上游序列相对于典型启动子而言，在两个方向上均能发挥作用。
J Biol Chem. 1988 Oct 15;263(29):14629-33.
9
Stereospecific positioning of the cis-acting sequence with respect to the canonical promoter is required for activation of the ompC gene by a positive regulator, OmpR, in Escherichia coli.在大肠杆菌中，顺式作用序列相对于典型启动子的立体特异性定位是由正调控因子OmpR激活ompC基因所必需的。
J Mol Biol. 1988 Aug 5;202(3):433-41. doi: 10.1016/0022-2836(88)90276-8.
10
Molecular analysis of mutant ompR genes exhibiting different phenotypes as to osmoregulation of the ompF and ompC genes of Escherichia coli.对大肠杆菌ompF和ompC基因渗透压调节表现出不同表型的ompR突变基因的分子分析。
Mol Gen Genet. 1986 Feb;202(2):194-9. doi: 10.1007/BF00331636.