乙型肝炎病毒复制与依赖于HBx的线粒体调节的细胞溶质钙水平升高有关。
Hepatitis B virus replication is associated with an HBx-dependent mitochondrion-regulated increase in cytosolic calcium levels.
作者信息
McClain Stephanie L, Clippinger Amy J, Lizzano Rebecca, Bouchard Michael J
机构信息
Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, PA 19102, USA.
出版信息
J Virol. 2007 Nov;81(21):12061-5. doi: 10.1128/JVI.00740-07. Epub 2007 Aug 15.
Abstract
The nonstructural hepatitis B virus (HBV) protein HBx has an important role in HBV replication and in HBV-associated liver disease. Many activities have been linked to HBx expression; however, the molecular mechanisms underlying many of these activities are unknown. One proposed HBx function is the regulation of cytosolic calcium. We analyzed calcium levels in HepG2 cells that expressed HBx or replicating HBV, and we demonstrated that HBx, expressed in the absence of other HBV proteins or in the context of HBV replication, elevates cytosolic calcium. We linked this elevation of cytosolic calcium to the association of HBx with the mitochondrial permeability transition pore.
摘要
乙型肝炎病毒（HBV）的非结构蛋白HBx在HBV复制及HBV相关肝病中发挥重要作用。许多活性都与HBx表达有关；然而，这些活性中许多的分子机制尚不清楚。一种提出的HBx功能是对胞质钙的调节。我们分析了表达HBx或复制HBV的HepG2细胞中的钙水平，并且我们证明，在不存在其他HBV蛋白的情况下表达的HBx或在HBV复制的背景下表达的HBx会升高胞质钙。我们将这种胞质钙的升高与HBx与线粒体通透性转换孔的关联联系起来。
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