Hidari K, Itonori S, Sanai Y, Ohashi M, Kasama T, Nagai Y
Department of Biochemistry, Faculty of Medicine, University of Tokyo.
J Biochem. 1991 Sep;110(3):412-6. doi: 10.1093/oxfordjournals.jbchem.a123595.
A monosialosylgangliopentaosyl ceramide was isolated from Xenopus laevis oocytes. It represented 5.8% of the total acidic glycosphingolipids. From the results of sugar-composition analysis, enzymatic hydrolysis, permethylation analysis, and negative ion fast atom bombardment mass spectrometry, the structure of the ganglioside was determined to be as follows: [sequence: see text] The predominant species of fatty acids were alpha-hydroxy fatty acids, h22:0, h24:0, and h24:1. The long chain bases of this ganglioside consisted mainly of d18:1 sphingosine and phytosphingosine. Other acidic glycolipids were also characterized. The most abundant component of acidic glycolipids was sulfatide, which represented 85.7% of the total acidic glycolipid mixture. GM3, GM2, GM1a, and GD1a were also detected.
从非洲爪蟾卵母细胞中分离出一种单唾液酸神经节五糖基神经酰胺。它占总酸性糖鞘脂的5.8%。通过糖组成分析、酶促水解、全甲基化分析和负离子快原子轰击质谱法的结果，确定该神经节苷脂的结构如下：[序列：见原文]主要的脂肪酸种类为α-羟基脂肪酸、h22:0、h24:0和h24:1。这种神经节苷脂的长链碱基主要由d18:1鞘氨醇和植物鞘氨醇组成。还对其他酸性糖脂进行了表征。酸性糖脂中最丰富的成分是硫苷脂，占总酸性糖脂混合物的85.7%。还检测到了GM3、GM2、GM1a和GD1a。
