Miwa H, Yamamoto M, Asano T
Faculty of Pharmaceutical Sciences, Fukuoka University, Japan.
J Chromatogr. 1991 Jul 17;568(1):25-34. doi: 10.1016/0378-4347(91)80337-c.
A novel high-performance liquid chromatographic method for biologically important fatty acids incorporated into platelet phospholipids in esterified form has been developed. 2-Nitrophenylhydrazine hydrochloride was used as a pre-column labelling agent to convert the saponified platelet phospholipids directly into corresponding fatty acid hydrazides, without a complicated isolation procedure. Isocratic separation was achieved within only 36 min for twenty-five saturated and mono- and polyunsaturated fatty acids (C8:0-C22:6), including cis and trans isomers, on a YMC-FA column. The analytical results showed good quantitative accuracy. Fatty acid compositions were determined in platelet phospholipids obtained from normal subjects and patients with diabetes mellitus. The method is simple, rapid and adequate for labelling esterified fatty acids in biological materials, and has several advantages with regard to resolution, analysis time and sensitivity over previously published methods.
已开发出一种新型高效液相色谱法，用于测定以酯化形式掺入血小板磷脂中的具有生物学重要性的脂肪酸。盐酸2-硝基苯肼用作柱前标记剂，可将皂化后的血小板磷脂直接转化为相应的脂肪酸酰肼，无需复杂的分离程序。在YMC-FA柱上，仅需36分钟就能实现对25种饱和脂肪酸、单不饱和脂肪酸和多不饱和脂肪酸（C8:0-C22:6）（包括顺式和反式异构体）的等度分离。分析结果显示出良好的定量准确性。测定了正常受试者和糖尿病患者血小板磷脂中的脂肪酸组成。该方法简单、快速，适用于标记生物材料中的酯化脂肪酸，在分离度、分析时间和灵敏度方面比以前发表的方法具有多个优势。
