Sharma M C, Joshi C, Das Gunjan
National Fellow Laboratory, Division of Medicine, Indian Veterinary Research Institute, Izatnagar 243122 UP, India.
Vet Res Commun. 2008 Jan;32(1):49-63. doi: 10.1007/s11259-007-9002-1. Epub 2007 Aug 14.
To evaluate the magnitude of copper deficiency in Northern India and to examine the various haematobiochemicals, enzymes, vitamins and immune functions affected by copper deficiency, and to identify the parameters which can be of diagnostic importance in copper deficiency, a survey was conducted in 12 districts of Northern India. Significant deficiency of copper was observed in soil, fodder and serum samples of buffalo heifers. Fifty hypocuperaemic buffalo heifers were selected from these areas and were randomly divided into two groups, A and B. The heifers in group A were provided with mineral mixture containing copper sulphate and in group B without copper sulphate. Significant (p<0.01) improvement in serum ceruloplasmin level was observed within 30 days of treatment, while significant (p<0.01) improvement in monoamine oxidase and liver cytochrome oxidase was observed at the 60th day of treatment in group A animals. Significant improvement was observed in T(3) and T(4), in the animals of group A within 60 days of treatment. The values of vitamin A and E showed significant (p<0.01) improvement within 30 days of treatment. The phagocytic activity of neutrophils against Candida albicans significantly (p<0.01) improved in group A within 60 days of treatment. Similarly, significant improvement in superoxide dismutase activity in red blood cells was observed at the 30th day, and in total leukocytes and whole blood at the 60th day in group A animals. Significant improvement in liver copper level was observed at the 30th day of treatment, while in group B the liver copper was significantly (p<0.01) depleted at the 60th day of experimentation. Additional copper supplementation improved growth performance significantly in group A.
为评估印度北部铜缺乏的程度，检测受铜缺乏影响的各种血液生化指标、酶、维生素和免疫功能，并确定对铜缺乏具有诊断意义的参数，在印度北部的12个地区进行了一项调查。在水牛小母牛的土壤、饲料和血清样本中观察到显著的铜缺乏。从这些地区挑选出50头低铜血症的水牛小母牛，并随机分为A、B两组。A组的小母牛被给予含硫酸铜的矿物质混合物，B组则不给予硫酸铜。治疗30天内，血清铜蓝蛋白水平显著（p<0.01）改善，而A组动物在治疗第60天时，单胺氧化酶和肝细胞色素氧化酶显著（p<0.01）改善。治疗60天内，A组动物的T(3)和T(4)显著改善。维生素A和E的值在治疗30天内显著（p<0.01）改善。治疗60天内，A组中性粒细胞对白色念珠菌的吞噬活性显著（p<0.01）改善。同样，A组动物在治疗第30天时红细胞中超氧化物歧化酶活性显著改善，在第60天时总白细胞和全血中超氧化物歧化酶活性显著改善。治疗第30天时肝脏铜水平显著改善，而在B组，实验第60天时肝脏铜显著（p<0.01）耗竭。额外补充铜显著改善了A组的生长性能。
