Sharma M C, Joshi Chinmay, Pathak N N, Kaur Harzit
Division of Medicine, Indian Veterinary Research Institute, Izatnagar 243122, UP, India.
Res Vet Sci. 2005 Oct;79(2):113-23. doi: 10.1016/j.rvsc.2004.11.015. Epub 2005 Mar 4.
A survey was conducted in 10 districts of Northern India to record the copper deficiency in soil, fodder and serum samples. Significant deficiency of copper was observed in soil, fodder and serum samples of heifers. The copper deficient animals were listless, showed depigmentation of skin, stiff gait and were anaemic and diahorric. The purpose of the study was to evaluate the magnitude of copper deficiency in Northern India and to examine the various haematobiochemicals, enzymes, vitamin and immune function which are affected by the deficiency of copper, so as to identify the parameters which can be of diagnostic importance in copper deficiency. Forty hypocuperemic heifers were selected from these areas and were randomly divided into two groups A and B. The heifers in group A were provided with mineral mixture containing copper sulphate and in group B without copper sulphate. Significant improvement (P<0.01) was observed in the haemoglobin (Hb), total leukocyte count (TLC) and total erythrocyte count (TEC) level at the 30th day of treatment in the animals of group A. Significant (P<0.01) improvement in serum. Ceruloplasmin (Cp) level was observed within 30 days of treatment, while significant (P<0.01) improvement in monoamine oxidase and liver cytochrome oxidase was observed at the 60th day of treatment in group A animals. Regarding hormones significant improvement was observed in T(3) and T(4), in the animals of group A within 60 days of treatment. The values of vitamin A and E showed significant (P<0.01) improvement within 30th days of treatment. The phagocytic activity of neutrophils against Candida albicans significantly (P<0.01) improved in group A within 60 days of treatment. Similarly significant improvement in superoxide dismutase activity in RBC was observed at the 30th day, and WBC and whole blood at 60th day in group A animals. Significant improvement in liver Cu level was observed at the 30th day of treatment, while in group B the liver Cu was significantly (P<0.01) depleted at the 60th day of experimentation. Additional Cu supplementation improved growth performance significantly in group A.
在印度北部的10个地区进行了一项调查，以记录土壤、饲料和血清样本中的铜缺乏情况。在小母牛的土壤、饲料和血清样本中观察到显著的铜缺乏。铜缺乏的动物无精打采，皮肤色素沉着减退，步态僵硬，贫血且腹泻。该研究的目的是评估印度北部铜缺乏的程度，并检查受铜缺乏影响的各种血液生化指标、酶、维生素和免疫功能，以便确定在铜缺乏诊断中具有重要意义的参数。从这些地区挑选了40头低铜血症小母牛，并随机分为A组和B组。A组小母牛给予含硫酸铜的矿物质混合物，B组不给予硫酸铜。在治疗第30天时，A组动物的血红蛋白（Hb）、白细胞总数（TLC）和红细胞总数（TEC）水平有显著改善（P<0.01）。在治疗30天内血清铜蓝蛋白（Cp）水平有显著（P<0.01）改善，而在治疗第60天时，A组动物的单胺氧化酶和肝细胞色素氧化酶有显著（P<0.01）改善。关于激素，在治疗60天内，A组动物的T(3)和T(4)有显著改善。维生素A和E的值在治疗第30天内有显著（P<0.01）改善。在治疗60天内，A组中性粒细胞对白色念珠菌的吞噬活性有显著（P<0.01）改善。同样，在治疗第30天时，A组动物红细胞中超氧化物歧化酶活性有显著改善，在第60天时白细胞和全血中超氧化物歧化酶活性有显著改善。在治疗第30天时肝脏铜水平有显著改善，而在B组，在实验第60天时肝脏铜显著（P<0.01）减少。额外补充铜显著改善了A组的生长性能。
