Verma Neelam, Kumar Kuldeep, Kaur Gurnoor, Anand Sneh
Biosensor Development Laboratory, Department of Biotechnology, Punjabi University, Patiala, India.
Artif Cells Blood Substit Immobil Biotechnol. 2007;35(4):449-56. doi: 10.1080/10731190701460358.
The present work aims at the development of a novel, diagnostic biosensor for monitoring asparagine levels in leukemia. Various immobilization strategies have been applied to improve the stability of the biocomponent (asparaginase). Response time studies have been carried out for different immobilization methods. Phenol Red indicator has been coimmobilized with asparaginase and color visualization approach has been optimized for various asparagine ranges. The detection limit of asparagine achieved with nitrocellulose membrane is 10(-1) M, with silicon gel is 10(-10)-10(-1) M, and with calcium alginate beads is 10(-9)-10(-1) M. Furthermore, the calcium alginate bead system of immobilization has been applied for the asparagine range detection in normal and leukemia serum samples.
本研究旨在开发一种用于监测白血病中天冬酰胺水平的新型诊断生物传感器。已应用各种固定化策略来提高生物组分（天冬酰胺酶）的稳定性。针对不同的固定化方法进行了响应时间研究。酚红指示剂已与天冬酰胺酶共固定，并且针对各种天冬酰胺范围优化了颜色可视化方法。用硝酸纤维素膜实现的天冬酰胺检测限为10^(-1) M，用硅胶为10^(-10)-10^(-1) M，用海藻酸钙珠为10^(-9)-10^(-1) M。此外，海藻酸钙珠固定化系统已用于正常和白血病血清样品中的天冬酰胺范围检测。
