Biosensor Research Laboratory, Department of Biotechnology, M. M. Modi College, Patiala, Punjab, India.
Artif Cells Nanomed Biotechnol. 2013 Jun;41(3):184-8. doi: 10.3109/10731199.2012.716062. Epub 2012 Sep 19.
The present work aims at the development of plant asparaginase-based asparagine biosensor for leukemia. It is a novel diagnostic for monitoring asparagine levels in patients suffering from acute lymphoblastic leukemia (ALL). Various immobilization strategies have been applied to improve the stability of the asparaginase. The latest and updated information including some new techniques of immobilization related to L-asparaginase such as gelatin, agarose, agar, and calcium alginate methods are described in detail along with response time studies and comparative data. Furthermore these immobilization techniques have been applied for the detection of asparagine in normal and leukemia serum samples.
本工作旨在开发基于植物天冬酰胺酶的用于白血病的天冬酰胺生物传感器。它是一种用于监测急性淋巴细胞白血病（ALL）患者天冬酰胺水平的新型诊断方法。已经应用了各种固定化策略来提高天冬酰胺酶的稳定性。本文详细描述了包括明胶、琼脂糖、琼脂和海藻酸钠方法在内的与 L-天冬酰胺酶相关的最新和更新的固定化技术信息，以及响应时间研究和比较数据。此外，这些固定化技术已应用于正常和白血病血清样品中天冬酰胺的检测。
