Barbolina Maria V, Stack M Sharon
Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL 60208, USA.
Semin Cell Dev Biol. 2008 Feb;19(1):24-33. doi: 10.1016/j.semcdb.2007.06.008. Epub 2007 Jul 10.
Enzymes in the matrix metalloproteinase (MMP) family have been linked to key events in developmental biology for almost 50 years. Biochemical, cellular and in vivo analyses have established that pericellular proteolysis contributes to numerous aspects of ontogeny including ovulation, fertilization, implantation, cellular migration, tissue remodeling and repair. Surface anchoring of proteinase activity provides spatial restrictions on substrate targeting. This review will utilize membrane type 1 MMP (MT1-MMP) as an example to highlight substrate diversity in pericellular proteolysis catalyzed by a membrane anchored MMP.
近50年来,基质金属蛋白酶(MMP)家族中的酶一直与发育生物学中的关键事件相关联。生化、细胞和体内分析已证实,细胞周围蛋白水解作用对个体发育的许多方面都有贡献,包括排卵、受精、着床、细胞迁移、组织重塑和修复。蛋白酶活性的表面锚定对底物靶向提供了空间限制。本综述将以膜型1 MMP(MT1-MMP)为例,突出由膜锚定MMP催化的细胞周围蛋白水解作用中的底物多样性。